凋亡促进因子TFAR19,Apaf-1与腰椎间盘突出症的关系研究

Relationship between Apoptosis Promoting Effector Molecules TFAR19,Apaf-1 and Lubar Intervertebral Disc Protrusion

目的探究凋亡促进因子TFAR19,Apaf-1与腰椎间盘突出症关系。方法随机选择广西医科大学第一附属医院脊柱骨病外科住院接受手术的腰椎间盘突出症患者99例,其中设单个节段突出的患者70例为A组,多个节段突出(腰椎间盘突出节段数量≥2个)的患者29例为B组,于入院次日收集外周静脉血。同时随机选取健康体检者40例作为对照组(C组)。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测腰椎间盘突出症患者血清中TFAR19,Apaf-1浓度。结果 C组、B组及A组血清中TFAR19浓度分别为1.30±0.09,1.85±0.14和2.33±0.25 ng/ml,各组之间TFAR19浓度差异具有统计学意义(F=7.979,P<0.01)。与C组相比,A组(q=3.60,P=0.012)、B组(q=5.59,P<0.01)TFAR19浓度差异具有统计学意义。A组、B组之间TFAR19浓度差异有统计学意义(q=2.93,P=0.012)。C组、B组及A组血清中Apaf-1浓度分别为107.52±11.58,159.22±11.87,203.20±20.21 Pg/ml,各组之间Apaf-1浓度差异具有统计学意义(F=8.828,P<0.01)。与C组相比,A组(q=3.89,P=0.007)、B组(q=5.86,P<0.01)Apaf-1浓度差异具有统计学意义。A组、B组之间Apaf-1浓度差异有统计学意义(q=2.97,P=0.037)。各组间性别构成比差异无统计学意义(x^2=0.229,P=0.892)。各组之间年龄差异无统计学意义(F=0.091,P=0.91)。结论腰椎间盘突出症患者血清TFAR19,Apaf-1表达上调加速了腰椎间盘退变过程,参与了腰椎间盘突出症的发生,并且与椎间盘突出节段数量相关,突出节段数量越多,TFAR19,Apaf-1表达量越高。

Objective To explore the relationship between apoptosis promoting effector molecules TFAR19,Apaf-1 and lubar intervertebral disc protrusion. Methods 99 of operation patients with lubar intervertebral disc protrusion in the First Affilia- ted Hospital of Guangxi Medical University Department of Spine and Ostcoputhy were recruited. Among them, single seg- ment of lubar intervertebral disc protrusion were 70 （Group A） ,more than one segments of lubar intervertebral disc protru- sion were 29 （Group B）. In addition,40 unrelated healthy people from physical examination center were enrolled as controls （Group C）. Enzyme-linked immunosorbent assay （ELISA） was used to examine serum TFAR19, Apaf-1 levels in lubar in- tervertebral disc protrusion patients. Results The level of TFAR19 in Group A,Group B and Group C respectively were 1.85±0.14,2.33±0.25 and 1.30±0.09 ng/ml. The TFAR19 activity in each group was statistically significant difference （F=7. 979,P〈0.01）. Compared with Group C,the TFAR19 activity in Group B （q=5.59,P〈0.01） ,Group A （q=3.60, P=0. 012） was statistically significant difference. The TFAR19 activity in lubar intervertebral disc protrusion patients sub- groups （Group A,Group B） was statistically significant difference （q=2.93,P=0. 012）. The level of Apaf-1 in Group A, Group B and Group C respectively were 159.22±11.87,203.20±20.21 and 107.52±11.58 pg/ml. The Apaf-1 activity in each group was statistically significant difference （F = 8. 828, P〈 0.01）. Compared with Group C, the Apaf-1 activity in Group B （q=5.86,P〈0.01） ,Group A （q=3.89,P=0. 007） was statistically significant difference. The Apaf-1 activity in lubar intervertebral disc protrusion patients subgroups （Group A, Group B） was statistically significant difference （q= 2. 97,P= 0. 037）. Male/female ratio between each groups was not statistically significant difference （x^2 = 0. 229, P = 0. 892）. Age between each groups was not statistically significant difference （F= 0. 091, P 〈 0.91）. Conclusion The aug- ment of TFAR19 and Apaf-1 promotes apoptosis of lubar intervertebral disc protrusion. It is connected with quantity of pro- trusive segments. The more segments of protrusion,the higher TFAR19 and Apaf-1 level of examination will be.