血管紧张素Ⅱ1型受体3个单核苷酸多态性与冠心病的相关性分析

Correlation between the Three Single Nucleotide Polymorphisms in Angiotensin Ⅱ Type one Receptor Gene and Coronary Artery Disease

目的观察中国汉族人群血管紧张素II 1型受体(AT1R)基因3个单核苷酸多态性(SNP)与冠心病(CAD)的相关性。方法提取2011年1月~2013年10月就诊于火箭军总医院的192例CAD患者和189例健康对照者外周血白细胞DNA和RNA,设计引物,应用荧光标记单碱基延伸及寡核苷酸微阵列芯片杂交技术检测AT1R基因rs6801836,rs2675511和rs5182的基因型。结果对照组和CAD组间3个SNP的基因型频率和等位基因频率差异无统计学意义(x^2=0.047~2.226,P值均>0.()5);由相互连锁的rs6801836和rs5182构成的单倍型以TT型为主,各单倍型频率在两组间差异无统计学意义(x^2=0.025~1.020,P值均>0.05)。结论研究中AT1R基因的3个SNP与CAD的易感性无关。

Objective To investigate the association between the three single nucleotide polymorphisms （SNP） in angiotensin II type one receptor （AT1R） gene with coronary artery disease （CAD） in Chinese Han nationality. Methods Extracted DNA and RNA samples of peripheral blood white cells from 192 CAD patients and 189 healthy individuals in Jan 2011 to Oct 2013 from the general hospital of the PLA Rocket Force. Designed primes and the three SNPs as rs6801836 ,rs2675511 and rs5182 of AT1R gene were analyzed with allele-specific fluorogenic oligonucleotide probes in an assay combining exten- sion and hybridization. Results The genotype and allele frequencies of the three SNPs were not significantly different be- tween the control group and CAD group （x^2 =0. 047~2. 226,all P〉0.05）. The major haplotype constructed with linkaged rs6801836 and rs5182 was TT. The frequency of every haplotypes showed no significantly difference between the two groups （x^2 =0. 025~1. 020,alt P〉0.05）. Conclusion The three polymorphisms of AT1R gene studied in this work showed no as- sociation with CAD susceptibility.