陕西省安康和延安两地区妇女HPV感染及型别分布对比研究

Comparative Study on HPV Infection and Hypotype Distribution between Ankang and Yan'an Area of Shaanxi Province

目的比较安康(陕南)和延安(陕北)两个不同地域人群感染HPV的特性,为宫颈癌的防治提供参考。方法采用PCR体外扩增和DNA反向斑点杂交技术对2012年7月~2016年7月安康市中心医院7 912例和延安医科大学附属医院3 543例妇女进行HPV基因分型检测,比较两地HPv感染人群年龄、检出率及型别分布。结果安康HPV总感染率为21.75%(1 721/7 912),延安为41.41%(1 467/3 543)。安康高危型感染阳性率为81.46%(1 402/1 721,包括混合高危感染),延安为86.16%(1 264/1 467);安康低危型感染阳性率为11.04%(190/1 721),延安为7.23%(106/1 467包括混合低危感染);安康高低危混合感染阳性率为7.50%(129/1 721),延安为6.61%(97/1 467)。两地>60岁组HPV感染率最高,安康为30.41%(104/342),延安为50%(60/120);两地常见高危型别为HPV16,58和52型,低危型别为HPV6.11和81型。结论两地HPV高发常见型别一致,但是延安HPV感染率显著高于安康(P<0.01),两地各年龄段人群均有较高感染率,原因有待进一步研究。

Objective Compare the HPV infection characters between women in different geographic area of Ankang （south of Shaanxi） and Yanan （north of Shaanxi）,to provide evidence based reference for cervical cancer control. Methods PCR amplification in vitro combined reverse dot-blot hybridization assay were used to analyze HPV genotypes of 7 912 cases from Ankang City Center Hospital,and 3 543 cases from Yanan Affiliated Hospital of Yanan Medical University from June 2012 to July 2016,and compared HPV infection rate,age and hypotype distribution of the cases. Results The HPV infection rate of Ankang area was 21.75G （1 721/7 912） ,and Yanan area was 41.41% （1 402/1 721）. The percentage of HPV positive women with high risk hypotype was 81.46%（1 402/1 721） in Ankang and 86.16% （1 264/1 467） in Yanan,with low risk hypotype was 11.04% （190/1 721） in Ankang and 7.23% （106/1 467） in Yanan,with high and low risk hypotype was 7.50% （129/147） in Ankang and 6.61% （97/1 467） in Yanan. The women more than 60 year old were higher HPV preva lence age group. The HPVinfection rate of this age group was 30.41% （104/342） in Ankang,and 50.00%（60/120） in Yanan. Yanan＇s HPV infection rate was obviously higher than Ankang. The popular HPV hypotypes in Yanan were same with that in Ankang. The most popular high risk hypotypes were HPV16,58 and 52,the most popular low-risk hypotypes were 6,11 and 81. Conclusion The HPV infection rate were rather high in all age group. The most popular HPV hypotypes were same between Ankang and Yanan area. But Yanan＇s HPV infection rate was obviously higher than Ankang （P〈 0.01）. The causes need further explore.