敦煌医方四时常服方对镉染毒大鼠骨和肾功能的影响

Effect of Dunhuang Sishichangfu Prescription on Bone and Renal Function in Rats Infected with Cadmium

目的:研究敦煌医方四时常服方对镉染毒大鼠骨和肾功能的影响。方法:将6组大鼠按照第一组(空白对照组)腹腔注射0.9%NaCl,其余五组(模型组、乙酰半胱氨酸组、四时常服方高中低剂量组)均腹腔注射0.1%氯化镉,1.5mg/kg注射(相当于1.5mL/kg),每周5次,共5周。染毒的同时,分别给予相应干预,乙酰半胱氨酸泡腾片溶液(5.4mg/mL)1mL/100g、四时常服方各种剂量(2.6g/mL,中1.3g/mL,低0.65g/mL)1mL/100g灌胃治疗,每天1次,连续5周。最后一次注射后,取血检测。结果:与空白对照组比较,模型组大鼠血清钙(Ca^(2+))、磷(P)、骨钙素显著降低,两组比较差异有统计学意义(P<0.05);与模型组比较,乙酰半胱氨酸组、四时常服方高中低剂量组大鼠血清钙、磷、骨钙素(OC)显著增加,差异有统计学意义(P<0.05)。与空白对照组比较,模型组大鼠血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶5b(TRACP-5b)、尿素氮(BUN)显著升高,两组比较差异有统计学意义(P<0.05);与模型组比较,乙酰半胱氨酸组、四时常服方高中低剂量组大鼠血清碱性磷酸酶、抗酒石酸酸性磷酸酶5b、尿素氮显著降低,差异有统计学意义(P<0.05)。结论:四时常服方对于镉染毒大鼠骨和肾功能具有一定的修复作用。

Objective：To study the Dunhuang Sishichangfu prescription on bone and renal function in rats infected with cadmium.Methods：The rats were divided into six groups.The first group was injected intraperitoneally with normal saline,the remaining five groups were injected intraperitoneally with 0.1% cadmium chloride,1.5 mg/kg,equivalent to 1.5 mL/kg,five times a week for 5 weeks.At the same time the rats were given corresponding intervention respectively：acetylcysteine effervescent tablets solution（5.4 mg/mL）1 mL/100 g,Sishichangfu prescription of the various doses（high 2.6 g/mL,medium 1.3 g/mL,low 0.65 g/mL）1 mL/100 g were given by gavage,once a day for five weeks.After the last injection,blood tests were taken.Results：Compared with the blank control group,the level of serum calcium（Ca2＋）,phosphorus（P）and osteocalcin（OC）were decreased significantly in the model group（P〈0.05）.Compared with the model group,the level of serum Ca2＋,P and OC were increased significantly in the acetylcysteine group and Sishichangfu prescription of high and low doses groups（P〈0.05）.Compared with the blank control group,serum alkaline phosphatase（ALP）,tartrate-resistant acid phosphatase 5b（TRACP-5b）and urea nitrogen（BUN）were significantly increased in the model group（P〈0.05）.Compared with the model group,the level of serum ALP,TRACP-5b and BUN were decreased significantly in the acetylcysteine group and Sishichangfu prescription of high and low doses groups（P〈0.05）.Conclusion：Sishichangfu prescription has a certain repair effect on bone and renal function in rats infected with cadmium.