CQMUH-011的抗炎作用研究

Investigation of anti-inflammatory effect of CQMUH-011

目的:观察和探讨CQMUH-011的抗炎作用及其机制。方法:采用二甲苯致小鼠耳肿胀、大鼠棉球肉芽肿和佐剂诱导的大鼠类风湿性关节炎3种模型,观察CQMUH-011对不同炎症模型的抗炎作用,通过耳肿胀度、棉球肉芽肿干重、关节炎指数、足围肿胀度和踝关节病理切片反映相应炎症的严重程度,并利用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测血清中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素6(interleukin-6,IL-6)的水平,相应的试剂盒检测一氧化氮(nitric oxide,NO)、丙二醛(malondialdehyde,MDA)和髓过氧化物酶(myeloperoxidase,MPO)水平。结果:新化合物CQMUH-011能明显降低小鼠耳肿胀度、大鼠棉球肉芽肿干重和踝关节肿胀度,减轻关节炎指数,缓解关节病理损伤;并降低血清中TNF-α、IL-6和NO浓度,减少MDA含量,抑制MPO活性。结论:新化合物CQMUH-011对炎症反应具有明显的抑制作用,这可能与其抑制炎症因子产生和抗脂质过氧化有关。

AIM： To observe the anti-inflammatory effect of CQMUH-011 and to explore its mechanism.METHODS： Three kinds of animal models,mouse ear swelling induced by xylene,rat granuloma induced by cotton ball and rat rheumatoid arthritis induced by Freund＇s complete adjuvant,were established to study the anti-inflammatory effect of CQMUH-011. The ear swelling degree,dry weight of cotton ball granuloma,arthritis index,paw swelling and ankle joint pathological changes were measured to reflect the severity of inflammation. The anti-inflammatory mechanisms of CQMUH-011 were investigated by detecting the serum levels of tumor necrosis factor-α（ TNF-α） and interleukin-6（ IL-6） by ELISA. Malondialdehyde（ MDA）,myeloperoxidase（ MPO） and nitric oxide（ NO） were determined by corresponding kits. RESULTS： Treatment with CQMUH-011 significantly decreased TNF-α,IL-6 and NO concentrations,MDA contents and MPO activity in the serum. Meanwhile,Ear swelling degree,dry weight of cotton ball granuloma,arthritis index,paw swelling and ankle joint pathological damage were attenuated. CONCLUSION： CQMUH-011 has an anti-inflammatory effect,which may be related to inhibiting the production of inflammatory factors and attenuating lipid peroxidation.