外周T细胞淋巴瘤患者中PD-1的表达对CD45RA^+、CD45RO^+T细胞的影响

The relationship between PD-1 expression and CD45RA^+ and CD45RO^+ T cells in peripheral T cell lymphoma patients

目的:探讨外周T细胞淋巴瘤(PTCL)患者淋巴瘤组织中PD-1的表达与外周血中初始和记忆T细胞水平的关系。方法:20例PTCL患者采用免疫组化法检测淋巴瘤组织中PD-1的表达,采用流式细胞术检测CHOP方案化疗前后外周血中CD4^+CD45RA^+、CD4^+、CD45RO^+、CD8^+CD45RA^+和CD8^+CD45RO^+T细胞的比例,分析PD-1的表达与T细胞亚群的关系。结果:PD-1、PD-L1蛋白在PTCL患者中表达升高,PD-1阳性患者疗效较差。PD-1阳性患者中CD4^+T细胞、CD8^+T细胞明显低于PD-1阴性患者;PD-1阴性组患者CD4^+CD45RO^+、CD8^+CD45RA^+、CD8^+CD45RO^+T细胞明显高于PD-1阳性患者,差异有统计学意义。结论:PTCL患者中存在PD-1/PD-L1蛋白的表达异常,PD-1阳性患者存在更明显的T细胞免疫功能缺陷。

Objective To investigate the relationship between the expression of PD -1 in lymphoid tissue and the level of initial and memory T cells in peripheral blood in peripheral T cell lymphoma （PTCL） patients. Methods： The expression of PD - 1 was detected by immunohistochemistry in lymphoma tissue in 20 PTCL patients. The proportion of CD4 ＋ CD45RA＋ ,CD4 ＋ CD45RO＋ ,CD8 ＋ CD45RA＋ and CD8 ＋ CD45RO ＋ T cells before and after CHOP chemo-therapy in peripheral blood in 20 PTCL patients were detected by flow cytometry. The relationship between expression of PD - 1 and T cell subsets was analysed. Results ： The expression of PD - 1 and PD - LI protein were increased in PTCL patients, and PD -1 positive patients had poor curative effect. The CD4 ＋ T cells and CD8 ＋ T cells in PD - 1 positive patients were significantly lower than that of PD - 1 negative group. The CD4 ＋ CD45RO ＋ , CD8 ＋ CD45RA ＋ and CD8 ＋ CD45RO ＋ T cells in the PD - 1 negative group were significantly higher than those in the PD - 1 positive group, the difference was statistically significant. Conclusion： The expression of PD - 1/PD - LI protein in patients with PTCL is abnormal, and the PD -1 positive patients have more obvious defects in T cell immune function.