5-FU有机金属框架纳米粒的体外细胞摄取及影响因素的研究

Preparation of 5-FU nanosized metal-organic framework and evaluation of their cell specificity

目的制备5-氟尿嘧啶(5-FU)有机金属框架(BTC)纳米粒,考察体外细胞摄取率及其影响因素。方法用室温配位调控法制备BTC纳米粒5-FU-Cu-BTC,用扫描电子显微镜观察其形态,用流式细胞仪测定其体外细胞摄取率及影响因素。结果 Cu-BTC载体的粒径约为100 nm,载药量为38%,体外细胞摄取实验表明:细胞摄取率与样品浓度和温度成正比。结论有机金属框架纳米粒可载入抗肿瘤药物并被肿瘤细胞摄取。

OBJECTIVE To prepare 5 - FU nanosized metal - organic framework and to evaluate their cell specificity and the influ- ence factors. METHODS 5 - FU nanosized metal - organic framework （ 5 - FU - Cu - BTC ） were prepared by coordination control method at room temperature. The mophorlogy of 5 - FU - Cu - BTC was observed by scanning electron microscopy. Flow cytometer （FCM） was performed to evaluate the cellular uptake and their influence factors. RESULTS The mean size of Cu - BTC was about 100 nm. The drug loading capacity was 38%. The cellular uptake experiment reveals that cellular uptake rate was proportional to the sample concentration and the incubation temperature. CONCLUSION Metal - organic framework nanoparticles is able to deliver antitumor drugs into tumor cells.