糖尿病伴乳腺癌患者硫氧还蛋白结合蛋白、前列腺跨膜上皮抗原2、转录因子家族中成员GATA3的表达及血清氧化应激指标的变化

Expressions of TXNIP,STAMP2 and GATA3 and the changes of serum oxidative stress indexes in diabetic patients with breast cancer

目的研究硫氧还蛋白结合蛋白(TXNIP)、前列腺跨膜上皮抗原2(STEAP2)、转录因子(GATA)家族中成员GATA3与糖尿病伴乳腺癌的关系,并分析患者术前及术后的血清氧化应激指标变化。方法检测45例糖尿病伴乳腺癌患者癌组织及对应癌旁组织中TXNIP、STAMP2、GAT.A3 mRNA表达量、蛋白水平及蛋白阳性表达情况,分析其临床病理特征,以及血清氧化应激指标的变化。结果乳腺癌组织中TXNIP mRNA、STAMP2 mRNA、GATA3 mRNA的相对表达量均下降(P<0.05)。TXNIP、STAMP2、GATA3蛋白在乳腺癌组织中的表达低于癌旁正常组织(P<0.05),且3个指标的表达阳性率为60%、66.7%、73.3%,均高于对应癌旁正常组织中的TXNIP蛋白表达阳性率(2.2%)(P<0.05)。乳腺癌组织中TXNIP、STAMP2、GATA3蛋白表达阳性率均与分化程度、淋巴结转移与临床分级相关(P<0.05)。患者术后血清MDA、髓过氧化物酶(MPO)均呈现先上升后下降变化趋势,SOD及总抗氧化力(TAC)呈现先下降后上升的变化趋势,术后6 d为转折点。结论 TXNIP、STAMP2、GATA3及血清氧化应激指标可能成为糖尿病伴乳腺癌患者诊断及治疗的参考指标。

Objective To measure the expressions of TXNIP, STAMP2 and GATA3 in diabetes patients with breast cancer and to determine the changes of oxidative stress indexes before and after surgery. Methods The expression levels of TXNIP, STAMP2 and GATA3 mRNA in cancer tissues and adjacent normal tissues were measured in 45 diabetes patients with breast cancer. The levels of serum oxidative stress indexes including MDA, MPO, SOD and TAC were detected and compared before and after surgery. Results The expressions of TXNIP,STAMP2 and GATA3 mRNA and their protein levels in cancer tissues were lower than those in adjacent normal tissues （P〈0. 05）. The positive expression rate of the three indexes were 60%, 66.7 % and 73.3 %, higher than that of TXNIP protein with 2.2% in the adjacent normal tissues （P〈 0.05）. The TXNIP, STAMP2 and GATA3 protein positive rate of breast cancer tissue were related to differentiation,lymph node metastasis and clinical grades （P〈0. 05）. Serum MDA and MPO levels increased first and then decreased. SOD and TAC showed a trend of decrease first and then increase. The turning point is 6 d after surgery. Conclusion TXNIP, STAMP2, GATA3 and serum oxidative stress indicators may be the indicators for diagnosis and treatment of breast cancer in diabetes patients.