冰川水和五脉绿绒蒿提取物的美容功效研究

Study on cosmetological efficacy of glacier water and Meconopsis quintuplinervia extract

研究了冰川水和五脉绿绒蒿提取物对人真皮成纤维细胞(HDF-a)的作用。采用MTT法检测了冰川水和五脉绿绒蒿提取物对HDF-a增殖活性的影响,利用荧光试剂盒检测了二者对HDF-a内ATP水平的影响,利用酶联免疫吸附试验(ELISA)法检测了二者对HDF-a细胞外基质中透明质酸含量的影响,利用Western Blot法研究了二者对HDF-a细胞外基质中Ⅰ型胶原蛋白和弹性蛋白表达的影响。实验结果表明,与对照组相比,冰川水处理后HDF-a增殖速度加快、代谢水平提高、透明质酸含量上升、弹性蛋白表达上调,且差异有统计学意义。五脉绿绒蒿提取物对HDF-a的作用在上述方面更为明显,同时可以促进Ⅰ型胶原蛋白的表达。二者共同作用时效果更为显著。

The effect of glacier water and Meconopsis quintuplinervia extract on human dermal fibroblast-adult （HDF-a） was studied respectively and collaboratively. Effect of glacier water and Meconopsis quintuplinervia extract on cell proliferation activity of HDF-a was evaluated by MTT assay and on level of cellular ATP （adenosine triphosphate） with an ATP Assay Kit. Effect of glacier water and Meconopsis quintuplinervia extract on content of hyaluronic acid in extracellular matrix （ECM） of HDF-a was evaluated by Enzyme-linked immunosorbent assay （ELISA） and their effect on expression of collagen I and elastin in ECM was detected by Western Blot. The results show that, as comparing with the control group, glacier water can promote the proliferation activity and energy metabolism level of HDF-a as well as enhancing cellular content of hyaluronic acid compared with the control group. Glacier water also displays a capacity of enhancing the expression of elastin. All of the above-mentioned differences are statistically significant. The effect of Meconopsis quintuplinervia extract with respect to the above-mentioned aspects is more obvious than that of glacier water, and the extract shows an ability of promoting the expression of collagen I. When glacier water and Meconopsis quintuplinervia extract are used collaboratively, their efficacy on HDF-a is much more obvious.