基于电化学方法检测花生油中黄曲霉毒素B1

Detection of aflatoxin B1 based on electrochemical method in peanut oil

基于黄曲霉毒素B1(AFB1)对乙酰胆碱酯酶(ACh E)的抑制作用,建立了ACh E电流型酶传感器,对食品中的AFB1进行快速检测。在最优条件下,AFB1质量浓度在2.00~7.00μg/m L范围内与传感器的响应呈线性关系,线性方程为A(%)=8.24057ρ+8.02743(%),检出限为0.89μg/m L。实际样品加标的平均回收率为96.3%~108.8%。方法适用于现场快速检测。

Agricultural products are abundant in china and food tradition is mainly based on grain, but a large number of agricultural products are often polluted by a variety of mycotoxins, especially aflatoxin B1 （ AFB1 ）. AFB1 have been widely concerned due to their high toxicity and carcinogenicity. In this study, the inhibitory effect of AFB1 on acetylcholinesterase （ACHE） was used to establish an AChE current type of enzyme sensor, which was used for rapid detection of AFB1 in food. Under optimal conditions, for the detection of AFB1, the as-prepared biosensor showed wide linear ranges of 2. 00 - 7.00μg/mL, with a linear equation of A （ % ） = 8. 24057p ＋ 8.02743 （ % ）, and the detection limit was 0. 89 μg/mL. The average recoveries of the actual samples was between 96. 3% - 108.8%. The method is simple, economical and suitable for field detection.