吉林人参低聚肽减轻大鼠慢性炎症作用

Oligopeptide of Panax ginseng originated from Jilin province attenuates chronic inflammation in rats

目的探讨吉林人参低聚肽减轻大鼠慢性炎症作用及机制。方法将大鼠随机分为5组,分别为对照组和62.5、125.0、250.0、500.0 mg/kg人参组,分别灌胃给予蒸馏水和相应浓度的吉林人参低聚肽,每天1次,连续30 d。采用棉球植入法计算棉球肉芽肿净重量、酶联免疫法检测大鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-10、一氧化氮(NO)和前列腺素E2(PGE2)水平。结果与对照组比较[(0.248±0.038)g],250.0 mg/kg人参组大鼠肉芽肿净重量[(0.162±0.047)g]下降(P<0.05),呈一定剂量效应关系;对照组大鼠血清中TNF-α、IL-1β、IL-10、PGE2水平及NO含量分别为(309.9±16.1)、(46.2±2.6)、(30.4±3.1)、(354.2±10.2)ng/L与(36.2±3.1)μmol/L,250.0 mg/kg人参组大鼠血清中TNF-α、IL-1β、IL-10、PGE2水平及NO含量分别为(202.4±17.3)、(21.4±2.4)、(68.4±3.9)、(438.6±16.3)ng/L与(31.5±2.5)μmol/L;与对照组比较,250.0mg/kg人参组大鼠血清中TNF-α、IL-1β与NO水平下降,IL-10、PGE2水平升高(P<0.05)。结论吉林人参低聚肽能够减轻大鼠慢性炎症反应,其机制可能与其降低促炎因子水平、升高抗炎因子水平有关。

Objective To explore anti-inflammatory effect of oligopeptitde extracted from Panax ginseng planted in Jilin province and the mechanism of the effect in rats with chronic inflammation. Methods Fifty specific pathogen free （SPF） Sprague-Dawley （SD） rats were randomly divided into 5 groups：a control group with gastric gavage of distilled water and four ginseng treatment groups with oligopeptide extracted from Panax ginseng planted in Jilin province at the dosages of 62. 5,125.0,250.0, and 500. 0 mg/kg via gastric gavage once a day for 30 consecutive days. Tampon granu- loma was induced in all the rats with tampon embedding method and granuloma was weighed. Enzyme-linked immu- nosorbent assay （ELISA） was adopted to detect serum inflammatory cytokines tumor necrosis factor-a （TNF-a）, inter-leukin 1 beta （IL-1β） ,interleukin 10 （IL-10） ,nitric oxide （NO） ,and prostaglandin E2 （PGE2） of the rats. Results The weight of tampon granulona of the rats exposed to 250 mg/kg oligopeptide from Panax ginseng decreased significantly compared to that of control rats （0. 162±0. 047 vs. 0. 248±0. 038 g,P 〈0. 05） ; in addition,compared to the control rats ,those exposed to 250 mg/kg oligopeptide showed significantly decreased serum TNF-a （202.4±17.3 vs. 309. 9±16.1 ng/L） ,IL-1β （21.4±2.4 vs. 46. 2 ±2.6 ng/L） ,and NO （31.5±2.5 vs. 36. 2±3. 1μmol/L） and increased IL-10 （68. 4±3.9 vs. 30.4±3. 1 ng/L） and PGE2 （438.6±16. 3 vs. 354. 2±10. 2 ng/L） （P 〈0.05 for all）. Conclu- sion Oligopeptide of Panax ginseng originated from Jilin province could attenuate chronic inflammation and the effect may relates to the decrease of pro-inflammatory factors and the increase of anti-inflammatory factors in rats.