PTEN蛋白在胃癌中的表达及临床病理意义

Expression of PTEN in gastric cancer and clinical pathological significance

目的研究10号染色体缺失的张力蛋白同源磷酸酶基因(PTEN)蛋白在胃癌中的表达与临床病理的关系,为寻找胃癌肿瘤标记物提供一定的实验依据。方法应用组织芯片及免疫组化方法检测140例胃癌组织、54例癌旁胃黏膜组织与64例正常胃黏膜组织中PTEN的表达情况,研究PTEN与胃癌发生发展的关系。结果PTEN蛋白在正常组织表达85.94%(55/64)明显高于癌前病变51.85%(28/54)与胃癌20.71%(29/140)(P<0.05),而癌前病变表达显著高于胃癌(P<0.05)。PTEN在高分化、中分化、低分化腺癌、黏液癌与印戒细胞癌分别表达44.83%(13/29)、26.08%(6/23)、14.58%(7/48)、7.40%(2/27)与7.69%(1/13),各组间差异具有显著性(P<0.05)。PTEN在淋巴结转移组表达11.53%(12/104)明显低于无淋巴结转移组47.22%(17/36)(P<0.05)。在Ⅰ+Ⅱ期表达38.30%(18/47)明显高于Ⅲ+Ⅳ期11.83%(11/93)(P<0.05)。结论 PTEN蛋白下调与胃癌的发生、分化程度、淋巴结转移和临床分期有关。

Objective To investigate the expression of PTEN protein in gastric cancer and clinical pathological significance. Methods Tissue microarray technique and SP Immunohistochemistry method were applied to detect PTEN protein expression in the 140 cases of gastric cancer,the 54 cases of tissue nearby and 64 cases matched non-cancer mucosa and evaluate the influence of abnormal expression of PTEN protein for carcinogenesis and progression of gastric cancer. Results Immunohistochemistry results revealed that the positive rates of PTEN protein expression in matched non-cancer mucosa, tissue nearby and gastric cancer were 83.36% （19/22） ,56.25% （27/48） and 24.44% （ 22/90）, respectively.The PTEN protein positive expression in gastric cancer were significantly lower than those matched non-cancer mucosa and the tissue nearby （P〈0.05）.The positive rates of PTEN expression were 44.83% （13/29） in well differentiated,26.08% （6/ 23） in moderate differentiated, 14.58% （7/48） in poorly differentiated, 7.40% （2/27） in mucinous cancer and 7.69% （1/13） in signet ring cell cancer, respectively, and the positive expression rates of PTEN between distinct degree of differentiation were significantly different in gastric cancer （P〈0.05）. Conclusion The down-regulation of PTEN protein was concerned with carcinogenesis, degree of differentiation,lymphatic metabasis and clinical stage in gastric cancer.