土家族药三百棒提取物对大鼠肢体缺血再灌注损伤血清AMPK与VEGF表达调控的影响

Effect of Tujia Medicine Toddalia Asiatic Extract on AMPK and VEGF Expressionin Rats with Ischemic-Reperfusion Injury

目的观察三百棒提取物对大鼠肢体缺血再灌注损伤血清腺苷酸活化蛋白激酶(AMPK)与血管内皮生长因子(VEGF)表达调控的影响。方法将66只SD大鼠随机分为对照组、缺血-再灌注组(IR组)和三百棒提取物干预组(T-IR组)。IR组30只,再分为再灌注后0h、2h、4h、8h、16h组,各6只;T-IR组30只,再分为再灌注后0h、2h、4h、8h、16h组,各6只;对照组6只。对照组不给药物,IR组于缺血前12h、缺血时和再灌注时各给予1 mL 0.9%氯化钠注射液灌胃;三百棒提取物干预组于缺血前12h、缺血时和再灌注时各给予1 mL三百棒提取物灌胃,分别于相应时间点在麻醉下抽取各组动物下腔静脉血,测量血清中AMPK和VEGF的含量。结果与对照组比较,IR组和T-IR组在再灌注后2h、4h、8h、16h大鼠血清AMPK水平均明显下降(均P<0.05)。与IR组各同期比较,T-IR组大鼠血清AMPK水平均明显上升(均P<0.05)。与对照组比较,IR组和三百棒提取物干预T-IR组在再灌注后4h、8h、16h大鼠血清VEGF水平明显升高(均P<0.05)。与IR组各同期比较,T-IR组大鼠血清VEGF水平均明显上升(均P<0.05)。结论三百棒提取物能增加大鼠肢体缺血再灌注损伤血清AMPK与VEGF表达,对大鼠肢体缺血再灌注损伤具有保护作用。

Objective： To investigate the effect of Tujia medicine Toddalia asiatica extract on AMPK and VEGF expression in rats with ischemic-reperfusion injury. Methods： 66 SD rats were randomly divided into control group,ischemia-reperfusion group and Toddalia asiatic extract intervention group. The control group animals were not given any drug;the ischemia-reperfusion group rats were gavaged with 1 mL of 0.9% NS in the first 12 h prior to ischemia,ischemia and reperfusion;the Toddalia asiatic extract intervention group rats were given 1 mL of Toddalia asiatic extracts in the first 12 h prior to ischemia,ischemia and reperfusion. Inferior vena venous blood was extracted respectivelyfrom each group animals at corresponding time points under anesthesia,the contents of serum AMPK and VEGF were measured. Results： Compared with the ischemia-reperfusion group,AMPK and VEGF content in serum of rats increased significantly in the Toddalia asiatic extract intervention group at each time point（P〈0.05）. The highestmeasured value appeared in 16 hours after reperfusion. Conclusion： Toddalia asiatic extract can increase serum AMPK and VEGF expression in rats with limb ischemia reperfusion injury,and has a protective effect on rat limb ischemia reperfusion injury.