锌锶共掺杂TiO_2多孔涂层的抗菌及生物相容性

Antibacterial and Biocompatibility of Zinc and Strontium Co-doped Porous TiO_2 Coating

为改善钛的抗菌性能和生物活性,采用微弧氧化(MAO)技术在纯钛表面制备了锌掺杂TiO_2涂层(M-Zn)、锶掺杂的TiO_2涂层(M-Sr)和锌锶共掺杂TiO_2涂层(M-Zn/Sr)。利用扫描电子显微镜(SEM)和X射线衍射仪(XRD)对制备的涂层的组织结构和成分进行分析;采用平板计数法研究了涂层的抗菌性能;利用细胞荧光染色和甲基噻唑基四唑(MTT)的方法探究了细胞在材料表面的生长状况。结果表明:形成的TiO_2涂层都是典型的多孔结构,主要由金红石和锐钛矿相组成,锌、锶的掺杂对涂层形貌影响不大。M-Zn/Sr涂层中锌、锶的原子数分数分别为7.9%和1.7%。M-Zn及M-Zn/Sr涂层大肠杆菌展现了良好的抗菌性能,抗菌率接近100%。M-Zn、M-Sr和M-Zn/Sr均能促进成骨细胞增殖,M-Zn/Sr涂层具有抗菌和细胞增殖的双重功能。

To improve the antimicrobial properties and biological activity of pure titanium, Zn-containing （M-Zn）, Sr- containing （M-Sr） and Zn/Sr co-containing （M-Zn/Sr） coatings were prepared by micro-arc oxidation technique. Scanning electron microscopy （SEM） and X-ray diffraction （XRD） were used to analyze the microstructure and phase composition of coatings. The antibacterial properties were assessed by a spread plate method. The vitality and proliferation of osteoblasts were evaluated by Live/Dead staining fluorescence and MTT method. The results indicate that all the coatings have porous structure with anatase phase and rutile phase, the doping of Zn and Sr has little effects on the coating morphologies. The contents of Zn and Sr in the M-Zn/Sr coating are 7.9% and 1.7%, respectively. M-Zn and M-Zn/Sr coatings show a good antibacterial properties against E. coli, and the antibacterialrates close to 100%. M-Zn, M-Sr and M-n/Sr coatings all can well support osteoblast proliferation. M-Zn/Sr coating has the dual function of antibacterial and cell proliferation.