桑不同药用部位HPLC指纹图谱比较研究

HPLC Fingerprints of Different Medicinal Parts of Morus alba L.

目的建立桑(Morus alba L.)不同药用部位HPLC指纹图谱,比较各药用部位化学成分的异同。方法采用高效液相色谱法,Agilent Eclipse XDB C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0m L·min^(-1),检测波长254 nm,柱温30℃。数据分析采用中药色谱指纹图谱相似度评价系统(2004A)软件。结果建立了桑不同药用部位的指纹图谱及共有模式图,桑白皮共有峰10个,桑枝共有峰11个,桑叶共有峰12个,桑椹共有峰8个。基于对照品比对和UPLC-Q-TOF/MS提供的精确相对分子质量对10个特征峰进行了指认。桑白皮、桑枝均含桑皮苷A、氧化白藜芦醇、桑酮G和桑辛素;桑叶、桑椹均含芦丁和异槲皮苷。结论该方法稳定、可靠、重复性好,明确了桑白皮、桑枝、桑叶、桑椹的基本组成,该研究结果为桑源药材的质量控制提供方法和理论依据。

OBJECTIVE To establish and compare the HPLC fingerprints of different medicinal parts of Morus alba. METHODS An HPLC analysis was performed on an Agilent Eclipse XDB Cls （4. 6 mm ×250 ram, 5 μm） chromatographic column, using gradi- ent elution with acetonitrile and 0. 1% aqueous formic acid at a flow rate of 1.0 mL · min -1. The column temperature was kept at 30℃, and the detection wavelength was set at 254 nm. The data was analyzed with Similarity Evaluation System for Chromatographic Fin- gerprint of Traditional Chinese Medicine （ Version 2004A）. RESULTS The HPLC fingerprints and common models of different me- dicinal parts of M. alba were established. The numbers of common peaks obtained in the fingerprints of Moil Cortex, Mori Ramulus, Moil folium, and Mori Fructus were 10, 11, 12, and 8, respectively. Ten characteristic peaks were identified by comparison with the reference substances and accurate molecular weights determined by UPLC-Q-TOF/MS. Moil Cortex and Mori Ramulus both had mul- berroside A, oxyresveratrol, kuwanon G, and morusin. Rutin and isoquercitrin were detected in both Mori folium and Moil Fructus. CONCLUSION The method is stable, reliable, and repeatable. The composition profiles of different medicinal parts are estab- lished, which provides a scientific basis for the quality control of M. alba.