大孔树脂分离纯化迷迭香叶总黄酮及抗氧化活性研究

Study on Separation,Purification and Antioxidant Activity of Total Flavonoids from Rosmarinus officinalis L. with Macroporous Resin

目的:探讨大孔树脂分离纯化迷迭香叶总黄酮及抗氧化活性。方法:选择6种类型大孔树脂,比较其吸附量、吸附率和解吸率,筛选最佳树脂,单因素分析最佳纯化工艺条件,检测迷迭香叶总黄酮体外抗氧化活性。结果:AB-8为最佳树脂,上样液浓度为2.25mg/mL,上样流速为3BV/h,pH为3.15,上样体积为1.5BV。以4BV 80%乙醇在流速2BV/h下洗脱,得黄酮的纯度为68.39%,精制倍数为3.37。迷迭香总黄酮对DPPH和ABTS自由基具有良好的清除能力。结论:AB-8树脂对迷迭香叶总黄酮具有良好的吸附和解吸效果,且迷迭香叶总黄酮具有良好的抗氧化作用。

Objective. Explore the separation, purification and antioxidant activity of total flavonoids from Rosmarinus officinalis L. with maeroporous resin. Methods： Six types of macroporous resins are selected to compare their performances in absorbing and desorbing flavonoids from Rosmarinus officinalis L.. The optimal purification conditions are analyzed by single-factor experiment. Results. The macroporous resin AB-8 is the best, the sample concentration is 2.25 mg/mL, the adsorption rate is 3 BV/h, the pH is 3.15, the sample volume is 1.5 BV, the eluting reagent is 4 BV 80M ethanol, the desorption rate is 2 BV/h, the dose of reagent is 4 times as much as the column volume. The purity of flavonoids is 68. 39%, and the purification factor is 3. 37. The total flavonoids from Rosmarinus officinalis L. have good antioxidant ability to DPPH and ABTS. Conclusion： The results show that AB-8 resin could be used for separation and purification of total flavonoids from Rosmarinus officinalis L. and has good antioxidant ability.