HPLC法同时测定食品中酸性橙Ⅱ和碱性嫩黄O的含量

HPLC simultaneous determination of acid orange Ⅱ and auramine O in foods

建立了同时检测食品中酸性橙Ⅱ和碱性嫩黄O两种非食用色素HPLC方法。样品经V(甲醇):V(水)=70:30超声提取,经C_(18)小柱净化后上样。色谱条件采用Kromasil C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相为V(甲醇):V(50 mmol/L乙酸铵)=50:50溶液,流速1.0 m L/min,检测波长450 nm,柱温35℃。结果表明:酸性橙Ⅱ和碱性嫩黄O在进样浓度2.5~12.5μg/m L内呈良好的线性关系,线性方程分别为Y=2×10~6X-1 240.1(r=0.999 9),Y=7×10~6X-5 284.2(r=0.999 6),二者检出限分别为0.120和0.032 mg/kg,在5类食品中的加标回收率分别为80.33%~90.47%和80.17%~102.64%。经方法学评价和抽样分析,该方法简便准确,重复性好,可作为食品安全检测中同时检测酸性橙Ⅱ和碱性嫩黄O参考方法。

A method of HPLC simultaneous determination of acid orange Ⅱ and auramine O in foods was established and optimized. The samples were ultrasonic extracted with solution of methanol： water（70：30）,and cleaned by C18 SPE column. The chromatographic conditions was Kromasil C18（4. 6 mm × 150 mm,5 μm）,mobile phase methanol-50mmol/L ammonium acetate（50：50）. The flow rate was 1. 0 m L/min,the detection wavelength was 450 nm,column temperature 35 ℃. The results showed the acid orange Ⅱand auramine O has a good linear relationship in the of 2. 5-12. 5 μg/m L,and the linear equations was Y = 2 × 10^6X-1 240. 1（ r = 0. 999 9） and Y = 7 × 10^6X-5 284. 2（ r = 0. 999 6）,with the limit of detection of 0. 120 mg/kg and 0. 032 mg/kg,respectively. The sample recoveries of five type food were 80. 33%-90. 47% and 80. 17%-102. 64%,respectively. Through the methodology evaluation and samples test. The method is simple and accurate with a good reproducibility. It can be used for simultaneous determination of acid orange Ⅱ and auramine O in foods.