美洲大蠊提取物对大鼠卵巢卵泡发育的影响

Effect of Periplaneta Americana Extract on Follicular Development of Ovary in Rats

实验旨在研究美洲大蠊提取物是否对大鼠卵巢卵泡发育产生影响。将成年SD大鼠随机分为3组,对照组(9 m L/kg,生理盐水)5只,低剂量组(9 m L/kg,0.03 g/m L美洲大蠊提取物)6只、高剂量组(9 m L/kg,0.09 g/m L美洲大蠊提取物)6只。每隔1 d灌胃一次,30 d后采集卵巢。结果表明:与对照组相比,低剂量组大鼠的卵巢重和卵巢体重比以及高剂量组大鼠的体重和卵巢重均显著增加(P<0.05);高、低剂量组大鼠卵巢原始卵泡的数目均显著减少(P<0.05),成熟卵泡显著增多(P<0.05);生长发育相关基因BMP4、GDF9的表达显著上调(P<0.05);低剂量组IGF-Ⅰ基因的表达有升高的趋势(P=0.058),但高剂量组则显著上调(P<0.05);3个组间凋亡相关基因Bcl-2、BAX、Fas、Fas L和Caspase-3的表达均无显著性差异;但高剂量组使增殖相关基因的表达有升高的趋势(P=0.06),使Ki67基因的表达显著升高PCNA(P<0.05)。本实验条件下,美洲大蠊提取物能够加快大鼠卵泡的发育,促进卵巢卵泡的成熟。

To study the effect of periplaneta americana extract on follicular development of ovary in rats. The adult SD rats were randomly assigned to three groups. The control group of 5 rats were only fi lled with physiological saline（9 m L/kg） through stomach perfusion. The 6 rats were used respectively in Low dose group（9 m L/kg, 0.03g/m L） and High dose group（9 m L/kg, 0.09 g/m L） which were fi lled with periplaneta Americana extract through stomach perfusion. Rats were treated every other day. After 30 days, the ovaries were collected. Compared with control group, Low dose group showed signifi cantly increased ovary weight and ovary weight relative to body weight, and High dose group showed signifi cantly increased ovary weight and body weight（P〈0.05）. High and low dose group all signifi cantly decreased the number of primordial follicles of rat ovaries（P〈0.05）, increased the number of graafi an follicles（P〈0.05）. High and low dose group signifi cantly up-regulated m RNA expression of GDF9 and BMP4（P〈0.05）. But the expression of IGF-gene tended to be higher in Low dose group（P=0.058）, and was up-regulated signifi cantly in High dose group（P〈0.05）. It had no signifi cant difference about the expression of Bcl-2, BAX, Fas, Fas L and Caspase-3 among the three groups. However, High dose group had significantly higher trend about the experssion of PCNA gene（P=0.06）, and significantly up-regulated the m RNA expression of ki67（P〈0.05）. Periplaneta americana extract can accelerate the development of ovarian follicles of rats, and promot the formation of graafi an follicles.