摘要
目的建立测定呋塞米片含量的超高效液相色谱(UHPLC)法。方法采用Shim-pack XR-ODSⅢ C_(18)柱,以水-四氢呋喃-冰醋酸(63∶37∶1)为流动相,检测波长为272 nm,流速为0.2 mL/min。结果回归方程为Y=6.92×10~6 X-2.88×10~4,r=0.999 9(n=6),进样量线性范围为0.019~0.761μg,平均回收率为100.90%,RSD为1.56%(n=6)。结论 UHPLC法在不影响分离效果的情况下可大大提高分析速度,改善分析效果,可以作为呋塞米片的质量控制标准。
Objective To establish a UHPLC method for quantity determination of Furosemide Tablets. Methods Shim-pack XR-ODSⅢ C18 column was adopted. The mobile phase consisted of water-tetrahydrofuran-glacial acetic acid （63 ：37 ：1）,the detection wavelength was 272 nm and the flow rate was 0. 2 mL/min. Results The regression equation was Y=6. 92 x 106 X-2. 88 x 104,and the furosemide concentration was linear in the range of 0. 019-0. 761 μg （r=0. 9999）,the average recovery was 100. 90%,RSD was 1. 56%（n=6）. Conclusion The UHPLC method can increase the analysis speed and improve analysis effect on the condition that separation effect had not been influenced. which can be used as one of the quality control measures for Furosemide Tablets.
出处
《中国药业》
CAS
2017年第4期19-21,共3页
China Pharmaceuticals
