TRPV1和TRPA1在大鼠睾丸痛模型背根神经节中的表达

Expressions of TRPV1 and TRPA1 in the dorsal root ganglion in the rat model of orchialgia

目的:探讨瞬时受体电位通道蛋白V1(TRPV1)和瞬时受体电位通道蛋白A1(TRPA1)在睾丸痛大鼠模型的表达及作用机制。方法:通过睾丸内注射2%醋酸的方法建立睾丸痛大鼠模型;造模后通过行为学实验检测大鼠自发痛计数与阴囊部热痛阈;RT-q PCR、Western印迹、免疫荧光检测T_(13)~L_1节段背根神经节(DRG)TRPV1和TRPA1的表达。结果:建模后大鼠自发痛计数较对照组明显增多[(22.63±3.42)次vs(0.13±0.35)次,P<0.01],热痛阈值下降,其下降峰值在注射后第4小时(4.85±1.00 s),与对照组(12.75±1.50 s)相比差异有统计学意义(P<0.01)。TRPV1和TRPA1均表达在DRG内神经元的胞膜上,与对照组相比,模型组大鼠DRG内TRPV1的mRNA水平上升至对照组的(1.77±0.34)倍,TRPA1的mRNA水平上升至对照组的(1.75±0.30)倍,且两种蛋白均表达上调,差异有统计学意义(P<0.05)。结论:TRPV1和TRPA1可能通过在DRG内表达上调,参与了睾丸痛大鼠模型的痛觉产生与外周痛敏的形成。

Objective ： To explore the expressions of transient receptor potential vanilloid 1 （TRPVI） and TRP ankyrin 1 （ TR- PAl ） in the dorsal root ganglion （DRG） and their action mechanisms in the rat model of orchialgia. Methods： The models of orchi- algia were established in male SD rats by injection of 2% acetic acid into the testis. Then the number of spontaneous pain responses and withdrawal latency in the model rats were recorded by behavioral tests and the expressions of TRPV1 and TRPA1 in Tl 3 - Ll DRGs determined by RT-qPCR, Western blot and immunofluorescence staining. Results ： Compared with the normal control rats, the orchi- algia models showed a significant increase in the number of spontaneous pain responses （0.13 ±0.35 vs 22.63 ± 3.42, P 〈0.01 ） and a decrease in the withdrawal latency at 4 hours after injection （ [ 12.75 ±1.50] vs [4.85 ± 1.00] s, P 〈0.05）. The mRNA expressions of both TRPV1 and TRPA1 were observed in the membrane of the neurons in the DRG, the former increased by 1.77 times and the latter by 1.75 times that of the control （P 〈0. 05）. Conclusion ： The expressions of TRPV1 and TRPA1 were up-regulated in the DRG of the rat models of orchialgia, which may be involved in the allodynia and hyperalgesia of the rats.