应激激活蛋白激酶及其磷酸化在晚期前列腺癌表达

Expressions of JNK and p-JNK in advanced prostate cancer and their clinical implications

目的:探讨应激激活蛋白激酶(JNK)与磷酸化JNK(p-JNK)在晚期前列腺癌(PCa)和良性前列腺增生(BPH)中的表达及意义。方法:收集晚期PCa组织蜡块40例,BPH组织蜡块21例。免疫组化法检测组织中JNK和p-JNK蛋白表达,并分析其与晚期PCa和BPH的关系,及与晚期PCa患者病理特征的关系。结果:BPH组与PCa组JNK蛋白阳性表达率分别为42.86%、52.50%,无统计学差异(P>0.05)。在未转移癌和转移癌、Gleason≤7和Gleason>7、PSA≤20μg/L和PSA>20μg/L、生存时间>5年和生存时间≤5年PCa中的JNK蛋白阳性表达率分别为53.85%和51.85%、58.82%和47.82%、57.14%和51.52%、60.00%和45.00%,无统计学差异(P>0.05)。p-JNK蛋白在BPH组与PCa组中的阳性表达率分别为33.33%、35.00%,无统计学差异(P>0.05)。在未转移癌和转移癌、Gleason≤7和Gleason>7、PSA≤20μg/L和PSA>20μg/L PCa中的p-JNK蛋白阳性表达率分别为30.77%和37.03%、35.29%和34.78%、43.75%和10.93%,无统计学差异(P>0.05)。生存时间>5年组pJNK阳性率为50%,生存时间≤5年组p-JNK阳性率为20.00%,有统计学差异(P<0.05)。结论:p-JNK高表达的PCa组生存时间长,并与PCa的预后相关。

Objective： To investigate the expressions of JNK and p-JNK in advanced prostate cancer （PCa） and benign prostatic hyperplasia （BPH） and their implications. Methods ： Using immunohistochemistry, we detected the expressions of JNK and p-JNK proteins in 40 cases of paraffin wax-embedded PCa and 21 cases of BPH tissues and analyzed their relationships with advanced PCa and BPH as well as with the pathologic features of advanced PCa. Results ： Statistically significant differences were not found in the positive expression rate of the JNK protein between BPH and PCa （42.86% vs 52.50%, P 〉 0.05）, non-metastatic and metastatic PCa （53.85% vs 51.85%, P 〉0.05）, Gleason ≤7 and Gleason 〉7 （58.82% vs 47.82% , P 〉0.05）, PSA ≤20 μg/L and PSA 〉20 μg/L （57.14% vs51.52%, P 〉0.05）, or survival 〉5 yr and survival ≤5 yr （60.00% vs45.00%, P 〉0.05）, nor in the expression level of p-JNK between BPH and PCa （33.33% vs 35.00% , P 〉0.05）, non-metastatic and metastatic PCa （30.77% vs 37.03%, P 〉0.05）, Gleason ≤7 and Gleason 〉7 （35.29% vs 34.78%, P 〉0.05）, or PSA ≤20 μg/L and PSA 〉20 μg/L （43.75% vs 10.93%, P 〉0.05）. However, the expression of p-JNK was significantly higher in the survival 〉5 yr than in the sur- vival ≤5 yr group of the PCa patients （ 50.00% vs 20.00%, P 〈 0.05 ）. Conclusion ： PCa patients with highly expressed p-JNK have a longer survival time and the high positive rate of p-JNK is associated with the prognosis of PCa.