姜黄素对阿霉素诱导H9C2心肌细胞损伤保护机制的研究

Protective Effects of Curcumin on Cell Injury induced by Doxorubicine in H9c2 Cell and Its Mechanism

目的研究探讨姜黄素对阿霉素诱导H9C2心肌细胞损伤保护作用的机制。方法本研究中阿霉素诱导的H9C2心肌细胞损伤模型是以8μmol/L的阿霉素作用于H9C2心肌细胞12h建立的。实验分正常组,阿霉素模型组,姜黄素低、中、高剂量组(10、20、40μmol/L),其中姜黄素低、中、高组先使用姜黄素预处理H9C2心肌细胞4h,后加入8μmol/L阿霉素共同孵育12h。MTT法测定细胞生存率;生化试剂盒检测MDA水平和SOD活性;Caspase-3活性检测试剂盒测定caspase-3活性。结果与阿霉素模型组比较,姜黄素低、中、高剂量组(10、20、40μmol/L)呈剂量性显著提高细胞存活率(P<0.05),降低细胞MDA水平和caspase-3活性(P<0.05);姜黄素中、高剂量组剂量性显著提高SOD的活性(P<0.05)。结论姜黄素能够通过提高细胞生存率和SOD活性,降低细胞MDA水平和caspase-3活性而对阿霉素诱导H9C2心肌细胞损伤起到抗氧化、抗凋亡的保护作用。

Objective To investigate the protective effects of curcumin on cell injury induced by Doxorubicine in H9C2 ceils and its mechanisms. Methods In this study H9C2 myocardial cell injury model was established by adriamycin and the concentration of adriamycin was 8μmoL/L. The experiments were divided into normal group, adriamycin group, curcumin low,medium and high dose group（10, 20,40 μmol/L）,In and low, medium and high curcumin group,H9C2 myocardial cell were pretreated with curcumin for 4h, after adding 8 mol/L adriamycin incubated for 12h. Cell viability was measured by MTr assay, MDA levels and SOD activity were detected by biochemical kit, and caspase - 3 activity was measured by Caspase - 3 assay kit. Results Compared with adriamycin group, low, the cell survival rate in the low medium and high curcumin dose group （ 10, 20, 40 mol/L） increased and decreased the level of MDA and activity of aspase -3 cells （P 〈 0.05） in a dose dependent manner; middle and high dose of curcumin enhanced the activity of SOD （P 〈 0.05）. Conclusions curcumin can increase the cell survival rate and SOD activity, and decrease the level of MDA and caspase- 3 activity of cells, which can protect against the injury of H9C2 myocardial cells induced by adriamycin.