氟对大鼠支持细胞紧密连接occludin基因与蛋白的影响

Effect of Fluorine on Sertoli Cell Tight Junction occludin Gene and Protein in Rat

试验旨在研究NaF对支持细胞occudin基因和蛋白表达的影响。采用不同浓度的NaF(0,0.1,1,10 mg/L)分别处理大鼠睾丸支持细胞48 h,q RT-PCR检测occludin基因水平的变化,免疫荧光技术检测occludin蛋白定位及水平的变化。结果显示,与对照相比,10 mg/L NaF染毒,occludin mRNA相对表达量显著下降(P<0.05);0.1,1.0 mg/L NaF染毒,occludin mRNA相对表达量下降,但差异不显著;occludin蛋白随着染氟剂量的增加,表达量逐渐降低,且occludin蛋白由细胞膜转移到细胞核附近的部位。说明NaF对支持细胞紧密连接的破坏可能与occudin基因与蛋白表达的变化有关。

The aim of this study was to figure out the effect of NaF on sertoli cell occludin gene and protein expression. Different concentrations of NaF （0, 0.1, 1.0, 10 mg/L） were used to treat rat testis sertoli cells for 48 h, then detected occludin gene changes by qRT-PCR, the protein changes by immunofluorescence technique. The result showed that the relatire expression of occludin mRNA decreased remarkbly（P 〈 0.05）, when the cell was treated with 10 mg/L NaF, compared with other groups. When the ceil was treated with 0.1, 1.0 mg/L NaF, the relative expression of occludin mRNA decreased, but the difference was not significant. And occludin protein expression gradually decreased and transferred from cell membrane to near the nucleus with the increasing dose of NaF. It illustrates that broken cell tight junction caused by NaF may be associated with the changes of occludin gene and protein expression.