多因素复合建立多发性脑梗死气虚血瘀证大鼠模型的探讨

Establishment of Rodent Model:Multiple Cerebral Infarction Combined with Qi-deficiency and Blood-stasis Syndrome in Rats

目的:通过多因素复合建立多发性脑梗死气虚血瘀证大鼠模型,研究多发性脑梗死气虚血瘀证大鼠模型的建立方法。方法:将大鼠随机分为假手术组、多发性脑梗死模型组(脑梗组)和3个病证结合模型组——力竭游泳复合多发性脑梗死组(游泳+脑梗组),睡眠剥夺复合多发性脑梗死组(睡夺+脑梗组),力竭游泳加睡眠剥夺复合多发性脑梗死组(游泳+睡夺+脑梗组)。3个病证结合模型组大鼠先分别采用相应因素(力竭游泳和睡眠剥夺)造模10 d,然后行多发性脑梗死手术,术后继续采用相应因素造模10 d;脑梗组大鼠只进行多发性脑梗死手术;术后第11天观察各组大鼠一般状态,采集中医证候指标(体重、抓力、脉搏、舌象等)和西医生化指标(血液流变学、凝血4项等)。结果:与假手术组比较,睡夺+脑梗组大鼠活动量减少、体重减轻、抓力下降,脉搏幅度减弱,舌面R,G,B值降低,全血黏度增加凝血酶原时间(PT)或活化部分凝血酶原时间(APTT)缩短(P<0.05,P<0.01),且较其他组指标变化明显。结论:通过中医证候指标和西医生化指标综合分析,睡眠剥夺复合多发性脑梗死的方法优于其他3个造模方法,更适合建立多发性脑梗死气虚血瘀证大鼠模型。

Objective： To establish a multifactor composite model with cerebral infarction with Qi- deficiency and blood-stasis syndrome in rats. Method： Rats were randomly divided into 5 groups, namely sham operation group, multiple cerebral infarction group, multiple cerebral infarction combined with exhaustive swimming group, multiple cerebral infarction combined with sleep deprivation group, multiple cerebral infarction combined with both exhaustive swimming and sleep deprivation group and sham group. Rats of the three syndromes combined model groups were provided with the corresponding factors （ sleep deprivation and exhaustive swimming） for 10 days. Then, the rats received multiple cerebral infarction surgery. After the surgery, sleep deprivation and exhaustive swimming were continuously implemented for another 10 days. The rats of multiple cerebral infarction group only received multiple cerebral infarction surgery. At the 11th day after the surgery, general status, body weight, grasping strength of fore limb, pulse, tongue picture, hemorheology and four items of blood coagulation were observed and assayed. Result： Body weight, grasping strength of fore limb, pulse intensity, and RGB value of tongue surface were decreased, while the whole blood viscosity increased, and prothrombin time （PT） and activated partial thromboplastin time （APTT） shortened in multiple cerebral infarction combined with sleep deprivation groups compared with sham operation group （P 〈 0.05, P 〈 0.01 ） , with more obvious changes in indexes than other groups. Conclusion： Through comprehensive analysis of traditional Chinese medicine syndromes （weight, grasping strength of fore limb, tongue, pulse） and western medicine biochemical indexes, the method of multiple cerebral infarction combined with sleep deprivation is superior to the other three modeling methods, and more suitable to establish a rat model with cerebral infarction with Qi-deficiency and blood-stasis syndrome.