摘要
The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells.The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C_(18) column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode.The mobile phase was consisted of methanol-water(0.1% formic acid)(55:45,V/V).The total run time was 5.0 min.The method was linear in the concentration range of 0.25-250.0 ng·mL^(-1).The lower limit of quantification was 0.25 ng·mL^(-1).The intra-and inter-day relative standard deviations of entire concentration range were less than 9.3%.The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.
The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells. The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C18 column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The mobile phase was consisted of methanol-water （0.1% formic acid） （55 ： 45, V/V）. The total run time was 5.0 min. The method was linear in the concentration range of 0.25-250.0 ng-mL^-1. The lower limit of quantification was 0.25 ng.mL^-1 The intra- and inter-day relative standard deviations of entire concentration range were less than 9.3%. The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.
基金
supported by Macao Science and Technology Development Fund(No.FDCT,006/2015/A1)
Open Project of Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards(No.Guizhongzhongkai201501)
