电针对心肌缺血大鼠模型心肌ATP敏感钾通道及蛋白激酶mRNA表达的影响

Effect of Electroacupuncture on ATP-sensitive Potassium Channel and Protein Kinase mRNA Expression in Myocardial Ischemia Model Rats

目的观察针刺不同经脉穴位对心肌缺血大鼠模型心肌细胞ATP敏感钾通道(Kir6.1、Kir6.2)及其结合蛋白(SUR2A、SUR2B)和蛋白激酶(PKA、PKG、PKCβ_2)mRNA表达水平的影响。方法健康雄性SD大鼠采用皮下多点位(四肢内侧根部和背部)注射ISO(85 mg/kg)制备心肌缺血模型后随机分成模型组、内关穴组、列缺穴组、非经非穴组(每组10只),另选择10只健康大鼠作为对照组。给予内关穴组、列缺穴组、非经非穴组大鼠相应电针干预,采用疏密波,强度2~3 m A,频率2~20 Hz,留针20 min,每日1次,连续7日。应用Real-time PCR检测左心室肌Kir6.1、Kir6.2及SUR2A、SUR2B、PKA、PKG、PKCβ_2 mRNA表达水平。结果与对照组比较,模型组各指标mRNA表达升高(P<0.01)。与模型组比较,内关穴组、列缺穴组各指标mRNA表达降低(P<0.01)。与内关穴组比较,列缺穴组、非经非穴组各指标mRNA表达升高(P<0.01);与列缺穴组比较,非经非穴组各指标mRNA表达升高(P<0.05)。结论电针内关穴可逆转缺血心肌细胞ATP敏感型钾通道(Kir6.1、Kir6.2)及其结合蛋白(SUR2A、SUR2B)与蛋白激酶(PKA、PKG、PKCβ_2)mRNA表达的变化。

Objective To observe the effect of electroacupuncture （EA） at different acupoints on mRNA expressions of ATP-sensitive potassium channel （Kir6.1, Kir6.2） and conjugated protein （SUR2A, SUR2B） and protein kinases （PKA, PKG and PKCβ2） in myocardial ischemia model rats. Methods Myocardial ischemia model was established in healthy male SD rats via subcutaneously injec- ting ISO （85 mg/kg） multipointedly （medial root of limbs and the back）. Then they were randomly divided into 4 groups, i.e., the model group, Neiguan （PC6） group, Lieque （LU7） group, non-acupoint group, 10 in each group. Besides, another 10 healthy rats were recruited as the control group. Corresponding EA was performed at respective acupoints to rats in Neiguan （PC6） group, Lieque （LU7） group, non-acu- point group, with dense-sparse wave, 2 -3 mA, 2 -20 Hz, needle retaining time of 20 min, once per day for 7 successive days. mRNA expression levels of Kir6.1 and Kir6.2, SUR2A, SUR2B, PKA, PKG, and PKCβ2 in left ventricular myocardium were analyzed by Real-time PCR. Results Compared with the con- trol group, mRNA expressions of each index increased in the model group （P 〈0.01 ）. Compared with the model group, mRNA expressions of each index significantly decreased in Neiguan （PC6） group and Lieque （LU7） group （P 〈0.01）. Compared with Neiguan （PC6） group, mRNA expressions of each index significantly increased in Lieque （LU7） group and non-acupoint group （P 〈0.01 ）. Compared with Lieque （LU7） group, mRNA expressions of each index significantly increased in non-acupoint group （P 〈0.05）Conclusion EA at Neiguan （PC6） could reverse mRNA expression changes of ATP-sensitive potassium channel （Kir6.1 and Kir6.2）and conjugated proteins （SUR2A and SUR2B） and protein kinases （PKA, PKG, and PKCβ2）.