温肾降浊散对大鼠阳离子化牛血清白蛋白肾小球肾炎模型的影响

The Influence of Spleen-Warming and Turbidity-Removing Powder on C-BSA Glomerulonephritis Model of Rats

目的:观察温肾降浊散对大鼠阳离子化牛血清白蛋白(Cationic bovine serum albumin,C-BSA)肾小球肾炎模型的影响。方法:随机选取10只为正常对照组,其余大鼠制备C-BSA肾小球肾炎模型,造模成功的大鼠随机分为模型对照组9只、雷公藤多苷治疗组8只、温肾降浊散高剂量组7只和温肾降浊散低剂量组9只。温肾降浊散高剂量组给予3.3 g·(kg·d)-1混悬液灌胃,低剂量组给予0.33 g·(kg·d)-1混悬液灌胃,每日1次。雷公藤多苷治疗组给予10 g·(kg·d)-1混悬液灌胃,每日1次。正常对照组和模型对照组灌胃给予等量蒸馏水,至实验第63天。取大鼠全血和尿液标本,分别做24 h尿蛋白定量、血尿素氮(blood urea nitrogen,BUN)、肌酐(serum creatinine,Scr)、三酰甘油(three acyl glycerin,TG)、血清总胆固醇(total cholesterol,TC)及低密度脂蛋白胆固醇(low density lipoprotein,LDL-C)的测定。结果:与正常对照组比较,模型对照组24 h尿蛋白、Scr、TC、TG、BUN和LDL均升高,其中24 h尿蛋白、Scr、TC和TG具有显著性差异(P<0.05),BUN、LDL无显著性差异(P>0.05)。与模型对照组比较,雷公藤多苷治疗组、温肾降浊散高剂量组及低剂量组24 h尿蛋白、Scr、TC、TG、BUN、LDL均降低,其中24 h尿蛋白、Scr、TC、TG具有显著性差异(P<0.05),BUN、LDL无显著性差异(P>0.05)。与雷公藤多苷治疗组比较,温肾降浊散高剂量组及低剂量组各指标均无显著性差异(P>0.05),作用与雷公藤多苷相当。结论:温肾降浊散对大鼠C-BSA肾小球肾炎模型有较好的治疗作用。

Objective： To observe the clinical curative effect of The Influence of Spleen-Warming and Turbidity-Removing Powder on cationic bovine serum albumin（ C-BSA） glomerulonephritis model of rats. Methods： Ten rats were randomly chosen into the normal control group,and the left ones were prepared to establish（ C-BSA） glomerulonephritis models. The successful model rats were randomly divided into the model group with 9 cases,the ripterygium wilfordii treatment group with 8 cases,Turbidity-Removing Powder high-dosage group with 7cases,and Turbidity-Removing Powder low-dosage group with 9 cases. The ones in TurbidityRemoving Powder high-dosage group were given suspension injection for gavage in the form of 3. 3 g·（ kg·d）-1,while the ones in Turbidity-Removing Powder low-dosage group were given suspension injection for gavage in the form of 0. 33 g·（ kg·d）-1,once daily. The ones in the ripterygium wilfordii treatment group were given suspension injection for gavage in the form of10 g·（ kg·d）-1,once daily. The ones Normal control group and model control group were given distilled water for sixty-third days. Then the whole blood and urine samples of rats were taken to determinate 24-h urine protein,blood urea nitrogen（ BUN）,serum creatinine（ Scr）,three acyl three glycerin（ TG）,serum total cholesterol（ TC） and low density lipoprotein cholesterol（ LDL-C） respectively. Results： Compared with the normal control group,the model control group＇s 24-h urinary protein,Scr,TC,TG,BUN and LDL were all increased; Among of them,the differences in 24-h urine protein,Scr,TC and TG had significant difference（ P〉0. 05）,but BUN and LDL had no significant difference（ P〉0. 05）. Compared with the model group,24-h urinary protein,Scr,TC,TG,BUN,LDL of the ripterygium wilfordii treatment group,Turbidity-Removing Powder high-dosage group,and Turbidity-Removing Powder low-dosage group were all decreased,Among of them,the differences in 24-h urine protein,Scr,TC and TG had significant difference（ P〈0. 05）,but BUN and LDL had no significant difference（ P〉0. 05）. Compared with the ripterygium wilfordii treatment group,each index had no significant difference in Turbidity-Removing Powder high-dosage group,and Turbidity-Removing Powder low-dosage group（ P〉0. 05）,and they were equivalent to tripterygium glycosides in functions.Conclusion： Spleen-Warming and Turbidity-Removing Powder has a remarkable clinical curative effect on C-BSA glomerulonephritis model of rats.