茯苓多糖提取工艺研究

Study on Extraction Technology of Polysaccharides from Poria Cocos

目的:优选茯苓水溶性粗多糖的水提醇沉工艺。方法:以茯苓水溶性多糖提取率为参察指标,以水为溶剂,选取粉碎力度、料液比、提取时间、提取次数、醇沉浓度、浓缩倍数、醇沉时间为影响因素,进行单因素考察,在综合单因素实验的基础上,采用L9(34)正交试验对水提和醇沉工艺进行优化设计,采用硫酸-蒽酮法测定多糖的含量。结果:茯苓水溶性多糖最佳的水提醇沉工艺为:过60目筛、料液比为1∶10,提取时间为100 min,提取1次,提取液浓缩至原体积的1/12,醇沉浓度为80%,静置12 h,过滤,滤饼用体积分数80%乙醇洗涤,60°C减压干燥。结论:此工艺成本低、操作简便、重现性好,可用于茯苓药材及饮片粗多糖的提取。

Objective：To optimize the water extract and ethanol precipitation process of water insoluble polysaccharides from Poria cocos.Methods：On the basis of single factor test with crushing,strength,materia liquid ratio,extraction time,extraction times and alcohol concentration,concentration ratio,alcohol sink for influence factors,the orthogonal test with 4 factors and 3 levels method was used to design and the content of the extract was determined using anthrone-sulfuric acid colorimetry method.Results：The results showed that the optimal extraction for obtaining the highest amounts of polysaccharides was carried out by over 60 mesh,water（1∶10）,with refluxing for 100 mins,the filtrat was concentrated to 1/12 V,then,ethanol was added to concentration of 80%,stewing 12 h,the precipitate filtated and washed with 80% ethanol,the product was dried under vacuum at 60° C.Conclusion：The process has low cost,simple operation and good reproducibility.It can be used for the extraction of crude polysaccharides from Poria cocos herbs and Yinpian.