ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性

Correlation between ATF5 protein expression level and drug sensitivity of gastric cancer AGS cells

目的:探究ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。方法:采用脂质体载体转染技术转染ATF5 pc DNA3.1质粒、ATF5 si RNA空质粒,构建ATF5蛋白高、中、低表达水平的AGS细胞组。Western blot实验检测AGS细胞中ATF5蛋白表达水平,MTT实验检测AGS细胞对紫杉醇、顺铂药物敏感性,平板克隆形成实验探讨AGS细胞集落形成差异,分析ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。结果:ATF5蛋白过表达组AGS细胞在紫杉醇、顺铂影响后存活率较对照组高,均P<0.05,IC50值较对照组也明显升高,均P=0.00;而低表达组存活率较对照组低,均P<0.05,IC50值较对照组也明显降低,P顺=0.00,P紫=0.002。过表达组细胞在药物影响后克隆形成数较对照组高,而低表达组克隆形成集落较对照组低,均P<0.05。结论:ATF5蛋白的过表达能降低胃癌AGS细胞对紫杉醇、顺铂的药物敏感性,而ATF5蛋白的低表达则能增加胃癌AGS细胞对紫杉醇、顺铂的药物敏感性,提示胃癌AGS细胞药敏水平可能与ATF5蛋白表达水平相关。

Objective To explore the correlation between ATF5 protein expression level and drug sensitivity of gastric cancer AGS cells. Methods We established the high, middle and low ATF5 expression groups by transfeeting the AGS cells with ATF5 pcDNA3.1 plasmid, ATF5 siRNA and empty plasmid using the liposome transfeetion technique. Then we detected the expression of the ATF5 protein of the 3 groups using the Western Blot. The drug sensitivity of AGS cells in 3 groups to paclitaxel and cisplatin was detected using the MTT experiments and plate clone formation experiments then the correlation between ATF5 protein expression level and drug sensitivity of gastric cancer AGS cells was analyzed. Results With the effect of paclitaxel or cisplatin on AGS cells in 3 groups, the surviving fraction and the IC50 of high ATF5 expression group were higher than those of control group, which indicated statistical significance （ all P 〈 0.05 ） ; while the surviving fraction and the IC50 of low ATF5 expression group were lower than those of control group （all P 〈 0.05, P eisplatin =0.00, P paclitaxel =0.002）. We found that cell clone formation of AGS cells to paclitaxel and cisplatin was significantly increased after the transfection with ATF5 protein plasmid and decreased after transfection with ATF5 siRNA plasmid （all P 〈 0.05 ）. Conclusion The expression of ATF5 protein is related to the drug sensitivity of gastric cancer AGS cells that upregulation of ATF5 protein expression can decrease the drug sensitivity of AGS cells to paclitaxel and cisplatin in gastric cancer.