单核细胞趋化蛋白3及微血管密度在皮肌炎和多发性肌炎患者肌肉组织中的测定及意义

Determination and significance of monocyte chemotactic protein-3 and microvessel density in muscular tissue of patients with dermatomyosits and polymyosits

目的:为检测皮肌炎(DM)和多发性肌炎(PM)患者骨骼肌中单核细胞趋化蛋白3(MCP-3)及CD34标记的微血管密度(MVD)的水平,进一步探究DM/PM的发病机制。方法:运用免疫组化法检测DM/PM患者组及对照组肌肉组织中的MCP-3及MVD的水平。结果:MCP-3在DM组和PM组患者肌肉组织中的阳性表达均明显高于对照组(P<0.001);DM组和PM组患者的肌肉组织中均可见CD34标记的微血管,且其MVD均高于对照组(P<0.001);MCP-3和MVD在DM/PM组患者肌肉组织中的水平差异均无显著性(P>0.05);DM组和PM组患者肌肉组织中的MCP-3与MVD水平的相关性均呈正相关(r分别为0.685、0.634,P<0.05)。结论:提示DM/PM患者肌肉组织中可能都有新生血管的生成,MCP-3可能参与了DM/PM新生血管的生成过程。

Objective To determine the levels of monocyte chemotactic protein- 3（MCP-3） and microvessel density （MVD） labeled by CD34 in skeletal muscle of patients with dermatomyositis （DM） and polymyositis （PM）, so as to investigate the pathogenesis of DM/PM. Methods The expression of MCP-3 and MVD were detected in DM/PM patients and the healthy controls by using the immnnohistochemical method. Results The positive expression rate of MCP-3 in DM group and PM group were higher than that in the control group （P 〈 0.001, respectively）. The microvessel marked by CD34 could be observed in muscle tissue of patients in DM group and PM group, and MVD in DM group and PM group was higher than that in the control group （P 〈 0.001 ）. No significant differences in the level of MCP-3 and MVD in the muscle tissue were found between the DM group and the PM group. The positive correlation between MCP-3 protein expression and MVD changes in the muscle tissue of patients inDM group and PM group was revealed （r = 0.685/0.634, P 〈 0.05）. Conclusion The muscle tissue of DM/PM patients may have a new blood vessel formation, formation in DM/PM.