摘要
采用超滤分离结合乙醇沉淀的方法,从刺芹侧耳下脚料水提物中纯化获得一多糖组分PEP30。苯酚硫酸法检测其糖含量约为94.2%,HPSEC-MALLS-RI系统分析其重均分子量(Mw)为3.74×106Da,多分散系数为1.03,为窄分布样品。通过红外光谱、单糖组成分析、甲基化GC-MS分析和核磁共振技术对多糖的结构特征进行了研究。结果表明,PEP30为一种β-D-葡聚糖,其主链以β-(1→3)-糖苷键连接,支链以β-(1→6)-糖苷键连接,支链与主链上糖残基的摩尔比为1:3。
Hot water extracts of Pleurotus eryngii fruiting body post-harvest wastes were separated by membrane ultrafiltration, and then treated with ethanol precipitation for obtaining a purified polysaccharide fraction PEP30. The total sugar content of PEP30 determined by phenol-sulfate method was 94.2%. HPSEC-MALLS-RI system was adopted to determine the molecular characteristics of this fraction. Analysis results indicated that PEP30 possessed high weight average molecular weight（Mw） of 3.74×106Da and small polydispersity of 1.03. Structural characteristics of PEP30 were distinguished by IR, monosaccharide composition analysis, methylation analysis and NMR technology. Results showed that PEP30 was a β-D-glucan, consisting of a backbone chain of β-（1→3）-linked-D-glucose residues, and one out of three being substituted at O-6 with single glucosyl residue.
出处
《菌物学报》
CAS
CSCD
北大核心
2017年第4期473-481,共9页
Mycosystema
基金
农业部公益性行业(农业)科研专项(201303080)
上海市农委重点攻关项目[沪农科攻字(2015)第6-1-6号]~~
关键词
食用菌
Β-葡聚糖
分离纯化
结构特征
edible fungi
β-glucan
separation and purification
structural characteristics
