BS^3交联分析β淀粉样蛋白的寡聚结构

BS^3 crosslinking analysis of the state of β-amyloid aggregate

使用交联剂双琥珀酰亚胺辛二酸酯磺酸钠盐(Bis(sulfosuccinimidyl),BS3)对Aβ1-42进行交联,通过与之前报道的戊二醛交联进行比较分析,可以看出BS3更适合分析蛋白的寡聚状态,这将为Aβ蛋白及类似的易聚集蛋白在体内、外研究提供很好的参考。

An agent named bis（sulfosuccinimidyl） （BS3） is used to cross-link Aβ1-42. Compared with glut- araldehyde which was previously reported as an Aβ cross-linking agent, it is not difficult to find that BS3 is more suitable for the analysis of protein in extra-membrane Aβ oligomers states, which will provide a good ex- perimental method to analyze Aβ protein and similar easy aggregation protein in vivo and vitro studies.