活动性系统性红斑狼疮患者外周血嗜碱性粒细胞CD62L、BAFF和CD40L表达变化及意义

Expression changes of CD62L,BAFF and CD40L in basophils of patients with systemic lupus erythematosus

目的探讨活动性系统性红斑狼疮(SLE)患者外周血嗜碱性粒细胞淋巴归巢受体(CD62L)、B淋巴细胞活化刺激因子(BAFF和CD40L)表达变化及意义。方法选取SLE患者57例,根据SLEDAI评分将SLE患者分为活动组(SLEDAI评分≥10分)29例和非活动组(SLEDAI评分<10分)28例,选取同期20例查体健康者作为对照组,应用流式细胞术检测SLE患者和正常人外周血CD62L、BAFF和CD40L表达,并采用Spearman法分析其与血清Anti-ds DNA水平的相关性。结果活动组与非活动组外周血CD62L表达水平均高于对照组(P均<0.05),且活动组高于非活动组(P<0.05)。活动组外周血BAFF和CD40L表达水平均高于对照组和非活动组(P均<0.05);而非活动组与对照组BAFF和CD40L表达差异无统计学意义(P均>0.05)。SLE患者外周血嗜碱性粒细胞CD62L和CD40L表达与血清Anti-ds DNA抗体水平均呈正相关(r分别为0.34、0.36,P均<0.05);而外周血嗜碱性粒细胞BAFF表达与血清Anti-ds DNA抗体水平无关(r=0.26,P=0.14)。结论活动性SLE患者外周血CD62L、BAFF和CD40L表达升高,嗜碱性粒细胞可能通过CD62L介导向次级淋巴器官迁移,通过BAFF和CD40L活化自身反应性B淋巴细胞而产生多种自身抗体,进而参与SLE的发病。

Objective To investigate expression changes of lymph homing receptor（ CD62L） and B-lymphocyte activation factors（ BAFF and CD40L） in peripheral blood basophils of patients with active systemic lupus erythematosus（ SLE） and their significance. Methods Fifty-seven SLE patients were selected and were divided into the active group（ SLEDAI score≥10,n = 29） and inactive group（ SLEDAI score 10,n = 28） according to the SLEDAI score. Twenty healthy volunteers were selected as the control group. The levels of CD62 L,BAFF and CD40 L in the peripheral blood basophils were detected by flow cytometry and their correlations with the Serum anti-ds DNA levels were analyzed by Spearman correlation analysis. Results The expression of CD62 L in peripheral blood basophils of the active group and inactive group was higher than that of the control group,and the active group was significantly higher than the inactive group（ P〈0. 05）. The expression of BAFF and CD40 L in peripheral blood basophils of the active group was significantly higher than that of the inactive group and control group（ all P〈0. 05）,while there was no significant difference between inactive group and control group（ P〉0. 05）. Expression of CD62 L and CD40 L in the peripheral blood basophils was positively correlated with serum anti-ds DNA levels in SLE patients（ r = 0. 34,r = 0. 36; all P〈0. 05）. The expression of BAFF in peripheral blood basophils was not related with serum anti-ds DNA levels in SLE patients（ r = 0. 26,P = 0. 14）. Conclusions Expression of CD62 L,BAFF and CD40 L in basophils of patients with active SLE increases. CD62 L may mediate the basophils migrating to the secondary lymph organs where the basophils could activate the self-reactive B-lymphocytes producing diverse antibodies through BAFF and CD40 L to participate in the pathogenesis of SLE.