Sysmex XT-4000i全血细胞分析仪在体液细胞计数及分类中的应用

Application of Sysmex XT-4000i hematology analyzer in cell count and cell differential count of body-fluid samples

目的评价Sysmex XT-4000i全血细胞分析仪体液模式在胸腹腔积液及脑脊液标本细胞计数及分类计数中的应用价值。方法采集2015年5月—9月住院患者胸腹腔积液及脑脊液标本共95份,应用Sysmex XT-4000i全血细胞分析仪(仪器法)和改良牛鲍计数板(手工法)分别计数,对两种方法计数结果进行比较。结果手工法与仪器法体液标本有核细胞计数及红细胞计数一致性较好(kappa=0.965,P<0.001;kappa=0.988,P<0.001);有核细胞分类计数中单个核细胞计数结果差异无统计学意义(P>0.05),而多个核细胞计数结果差异有统计学意义(P<0.05)。结论全血细胞分析仪体液模式精密度高,效率高,易于大批量操作,可能替代部分物理性状较好的常规胸腹腔积液和脑脊液标本的细胞计数,但细胞分类计数结果还需要显微镜检查辅助。

Objective To evaluate the value of Sysmex XT-4000i hematology analyzer in its body-fluid mode in cell count and cell differential count of pleural effusion, ascites and cerebrospinal fluid samples. Methods A total of 95 pleural effusion, ascites and cerebrospinal fluid samples were collected from patients hospitalized between May and September 2015. The samples were tested by Sysmex XT-4000i hematology analyzer （instrument method） and modified Neubauer hemocytometer （manual method） for cell count, and the results of them were compared and analyzed. Results The instrument method and the manual method had a good consistency in nuclear cell count and erythrocyte count （kappa=0.965, P〈 0.001; kappa=0.988, P〈0.001）. There was no significant difference in the count of mononuclear cells （P〉 0.05）. However, there was a significant difference in the count of multiple nuclear cells （P〈0.05）. Conclusions Hematology analyzer in its body-fluid mode may replace manual method in cell count of pleural effusion, ascites and cerebrospinal fluids for its high precision, high efficiency and easy operation. However, cell differential count of this method needs microscopic examination assistance.