UAE鲍鱼糖蛋白工艺优化及HPLC测其葡萄糖含量

The Process Optimization of Extracting Glycoprotein Assisted Ultrasonic from Abalone and Determination of Glucose Content

以三亚市鲍鱼肌肉为原料,采用超声辅助提取工艺,研究了提取溶剂、超声功率、料固比、提取时间和提取温度等因素对糖蛋白提取效果的影响,通过设计正交试验分析,确定最佳工艺条件为:料液比为1︰18(g/mL),超声浸提时间为20 min,超声功率为960 W,氯化钠溶液浓度为0.2 mol/L,鲍鱼肌肉糖蛋白提取率为0.51%,葡萄糖的含量为0.702 5 mg/kg,与传统提取方法相比超声提取能有效降低提取时间,提高提取效果。

To research the effect on glycoprotein extraction of extraction solvent, ultrasonic power, liquid-solid ratio, extraction time and extraction temperature, the author has taken abalone muscle as raw materials from Sanya, and used ultrasonic assisted extraction technology and orthogonal experimental analysis. The best process conditions： liquid-solid ratio 1 ： 18 （g/mL）, ultrasonic extraction time 20 min, ultrasonic power 960 W, NaCl solution concentration 0.2 mol/L, abalone muscle glycoprotein extraction efficiency 0.51%, glucose content 0.702 5 mg/kg. Comparing with conventional method, ultrasonic extraction can effectively reduce the extraction time, and increase extraction effect.