大越豆芋块根提取物抗氧化及海虾幼虫致死活性的研究

Antioxidant and Lethal Activity on Brine Shrimp of Extracts from Root of Apios americana Medikus

研究大越豆芋块根提取物抗氧化与海虾幼虫致死活性。采用石油醚、乙酸乙酯和正丁醇对大越豆芋块根醇提物进行分极萃取,通过比色法测定醇提物及4个极相的总酚、总黄酮及总皂苷含量,DPPH法、ABTS+法、FRAP法及海虾幼虫致死生物活性法测定醇提物及4个极相的抗氧化能力和细胞毒活性。结果表明,大越豆芋块根醇取物乙酸乙酯相的有效成分含量(总酚24.15%、总黄酮10.25%、总皂苷10.99%,占提取物质量分数)及海虾幼虫致死活性(LC50值为315.40μg/m L)最高,其清除自由基能力(对DPPH及ABTS+自由基的IC50值分别为80.12μg/m L和25.42μg/m L)和亚铁离子还原力均显著高于醇提物及其它分极组分(p<0.05)。乙酸乙酯相为大越豆芋块根的主要活性部位,可供进一步开发利用。

Methanol extract from root of Apios american Medikus was extracted with petroleum ether, ethyl acetate and n-butanol successively. The total saponins, polyphenols and flavonoids content of the different polar extracts were measured by colorimetric method. The antioxidant capacities were detected by DPPH, ABTS＋and FRAP method. The lethal activity was detected by the lethal-to-prawn larva bioactivity experiment. The results showed demonstrated ethyl acetate extract had the most total polyphenols （24.15%）, flavonoids （10.25%） and saponins （10.99%）, and its lethal effect on brine shrimp was the best （LC50=315.40μg/mL）. Besides, the radical scavenging capacities （IC50 of DPPH and ABTS＋mdicals were 80.12μg/ mL, 25.42μg/mL, respectively） and the FRAP value were significantly stronger than other different polar extracts. It was also demonstrated that the ethyl acetate pole from root of Apios americana Medikus was the main active part, which was available for further development and utilization. Correlation analysis indicated that antioxidant substances were mainly flavonoids, while polyphenols are the main component of cytotoxic activity.