摘要
为探究荧光素酶编码基因表达产物发光特性,以青海弧菌基因组为模版扩增得到luxCDABE基因,构建表达载体pHSG396-luxCDABE。将载体导入大肠杆菌Top10,成功获得表达菌株Top10/pHSG396-luxCDABE(T-pluxCDABE),测定重组菌株的表达活性,与实验室构建的菌株Top10/pHSG 396-luxAB(T-pluxAB)发光情况进行比较。
In order to further explore luminescent property of Vibrio qinghaiensis luciferase,the luxCDABE gene coding Vibrio qinghaiensis luciferase and related enhanced bioluminescent was amplified by using the genomic DNA of Vibrio qinghaiensis as the template in this research.The lux gene was subcloned into expression vector pHSG396 and its product lux was overexpressed in Escherchia coil Top10.Luminescent property of the Top10/pHSG396-1uxCDABE that was obtained in this study was compared with engineering bacteria Top10/pHSG396-1uxAB constructed in early study.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第2期206-211,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(No.31172236)
中央高校基本科研业务费(No.QN2011138)
