6个猪品种CD53基因中结构变异SV184分布频率的研究

Study on Distribution Frequency of Structure Variation SV184 in CD53 Gene of Six Pig Breeds

基于香猪全基因组重测序数据,在白细胞表面抗原CD53基因中筛选到一个结构变异(structure variant,SV)184,为了探索不同猪品种间此结构变异是否存在多态性变化,本试验选择香猪、大白猪、糯谷猪、柯乐猪、江口萝卜猪和荣昌猪作为试验动物,采用PCR方法对猪群中SV184的分布频率进行比较研究,应用RT-PCR技术,分析CD53基因的原初转录本序列结构,研究SV184对CD53基因的转录是否有直接影响。结果显示,6个猪品种中SV184的分布频率存在明显差异,香猪以VN基因型为主,其他5个猪品种是NN基因型占优势;计算两种等位基因的频率,香猪以缺失的V等位基因为主,其他5个猪品种以正常的N等位基因为主。选择3种基因型个体的血液样本,逆转录后分析CD53基因的原初转录本的序列,从中检测到缺失型和不缺失的两种转录本形式,提示SV184对CD53基因的转录无直接影响。SV184可作为分子标记用于区分香猪和其他猪品种。

A candidate structure variation （SV） 184 was screened out from the resequencing data of Xiang pig. To explore the polymorphism of SV184 among pig breeds, the genotype distributions of SV184 in leukocyte antigen protein （CD53） gene was detected by PCR method taking six Chinese and European pig breeds as samples including Xiang pig, Large White pig, Nuogu pig, Kele pig,Jiangkouluobo pig and Rongchang pig breeds. Furthermore, the primary transcripts of three genotypes of SV184 were determined using RT-PCR technology. Results showed that the type VN was dominant in Xiang pig, while type NN was mainly in others. Both of two primary transcripts of CD53 gene were determined from the heterozygous genotype of SV184,which indicated that the presence of SV184 might not disturb the template selection in the process of CD53 gene transcription. It suggested that SV184 could be taken as one of molecular marker to distinguish Xiang pig from other pig breeds.