Zein/鼠尾Ⅰ型胶原纳米纤维膜的制备与表征

Preparation and characterization of Zein/Collagen Ⅰ nanofiber membrane

为制备仿细胞外基质支架材料,采用静电纺丝技术制备了玉米醇溶蛋白/鼠尾Ⅰ型胶原纳米纤维膜,研究了胶原含量对其纤维直径、亲疏水性、力学性能和生物相容性的影响.结果表明:胶原的最佳添加质量分数为26.7%.胶原的加入能够减小纤维直径,改善其亲水性,且提高其断裂伸长率,有利于更好地模拟细胞外基体组织结构;纤维膜毒性等级为1级,不存在潜在的细胞毒性;接种L929细胞0.5 h后细胞黏附率达60%以上,并且可以促进细胞增殖生长.实验说明该纳米纤维膜具有良好的生物相容性,可作为仿细胞外基质应用于组织工程领域.

In order to prepare the extracellular matrix scaffold material, the Zein/Collagen I nanofiber membranes were pre-pared through electrospinning. The effects of collagen content on the fiber diameter, hydrophobicity, mechanical properties and biocompatibility were carefully investigated. The results showed that the best addition amount of collagen was 26.7%. The addition of collagen could decrease the fiber diameter and improve its hydrophilicity, and increase the elongation at break, which was favorable to simulate the extracellular matrix. The toxicity grade of the membrane was grade 1, and there was no potential cytotoxicity. Furthermore, the cell adhesion rate of L929 cells was more than 60% within 0.5 h, and it could promote the cell proliferation, which indicates that the nanofiber membrane have good biocompatibility and can be used as an extracellular matrix in the field of tissue engineering.