麻芍平喘汤对哮喘大鼠气道重塑及TGF-β1、PDGF-BB的影响

The impact of the Mashao Pingchuan decoction to the airway remodeling of asthma rats and TGF-β1,PDGF-BB

[目的]探讨麻芍平喘汤对哮喘大鼠气道重塑及转化生长因子(TGF-β1)、血小板源性生长因子BB型(PDGF-BB)的影响。[方法]将70只SD大鼠随即分为正常组(A)、模型组(B)、麻芍平喘低剂量组(C)、麻芍平喘中剂量组(D)、麻芍平喘高剂量组(E)、地塞米松组(F)、阳和平喘组(G)。卵白蛋白(OVA)多点致敏激发大鼠制作哮喘气道重塑模型,在实验第28~42天,正常组与模型组分别予生理盐水灌胃,其余各组予相应药物干预。实验结束后HE染色观察哮喘大鼠肺组织病理形态改变及气道形态学变化;免疫组织化学染色法检测支气管肺组织TGF-β1、PDGF-BB的蛋白表达。[结果]麻芍平喘汤能够减轻哮喘大鼠气道炎性细胞浸润及气道重塑;与A组比较B组大鼠TGF-β1、PDGF-BB的蛋白含量均明显升高(P<0.01);与B组相比,中药组和西药组TGF-β1、PDGF-BB水平明显降低(P<0.01);与F组相比,E组TGF-β1、PDGF-BB水平降低明显(P<0.05);与G组相比,E组效果更好(P<0.01)。[结论]麻芍平喘汤能延缓哮喘气道重塑,控制炎症,其中TGF-β1、PDGF-BB与气道重塑呈正相关。

[Objective] To study the impact of Mashao Pingchuan decoction on TGF-β1 and PDGF-BB in the airway remodeling of asthma rats. [Methods] The 70 male SD rats were randomly divided into normal group, model group, Mashao Pingchuan decoction low, medium and high dose group, dexamethasone group and Yanghe AsthmaEasing group.To use the ovalbumin（OVA）more sensitization inspire making airway remodelling of asthma model rats, in experiment of 28~42 days, the model group and normal group respectively for the physiological saline lavage,the rest of the group to the corresponding drug intervention. After the experiment, HE dyeing observe the changes of asthma rat lung tissue pathology morphology and airway remodelling; immunohistochemistry to detect the protein expression of the bronchial lung tissue TGF-β1, PDGF-BB. [Results] Mashao Pingchuan decoction could reduce the airways of the asthma rat inflammatory cells infiltration and airway remodeling; group B compared with group A rats TGF-β1 and PDGF-BB were significantly increased（P〈0.01）; compared with group B, the TCM group and western medicine group TGF-β1 and PDGF-BB levels significantly decreased（P〈0.01）; compared with group F,E group TGF-β1 and PDGF-BB levels decreased（P〈0.05）; compared with G,group E is better（P〈0.01）.[Conclution] Mashaopingchuan decoction can delay the airway remodeling of asthma, control inflammation, and TGF-β1, PDGF-BB was positively related with airway remodeling.