摘要
该研究采用Illumina Hi-Seq2500高通量测序技术,对叶背有纤维和无纤维发育的两组钩苞大丁草(Gerbera delavayi Franch.)叶片样品的cDNA进行转录组测序,分析其叶背毡毛纤维发育机理。测序结果得到了108 694条单基因序列,进一步筛选得到了1 605条差异表达基因,838条差异表达基因在GO数据库具有功能定义,512条差异表达基因在COG分类体系中具有详细的蛋白功能释义,315条差异表达基因注释到了KEGG数据库中。其中,氨基糖和核苷酸糖代谢途径中控制纤维素合成的相关基因,苯丙烷类生物合成途径中控制木质素合成的相关基因,以及激素信号转导途径中控制生长素信号转导的相关基因表达量下调;激素信号转导途径中控制细胞分裂素、脱落酸信号转导的相关基因表达量上调。研究结果在一定程度上丰富了钩苞大丁草的基因信息,并为后续的遗传改良提供了基础数据。
In the present study,two samples（abaxial leaves with and without penniform fiber）＇s cDNA were sequenced based on Illumina Hi-Seq2500 to analyze the fiber development mechanism.The results indicated that 108 694 unigenes were obtained.Then,1 605 differential expressed genes（DEGs）were selected.830 DEGs were divided into 39 GO terms,512 DEGs were assigned to 25 categories with COG database.Function annotation against KEGG database obtained 315 DEGs.10significantly reliable enrichment pathways were selected by enrichment analysis of 79 KEGG pathways.DEGs in ＂amino sugar and nucleotide sugar metabolism＂which control cellulose biosynthesis,DEGs in ＂phenylpropanoid biosynthesis＂which control lignin biosynthesis and DEGs in ＂plant hormone signal transduction＂which control auxin＇s signal transduction were down-regulated.While those in ＂plant hormone signal transduction＂which control cytokinin and abscisic acid signal transduction were up-regulated.These results greatly en-riched genetic information of G.delavayi and provided basic data for function verification and genetic improvement of fiber traits in the future.
出处
《西北植物学报》
CAS
CSCD
北大核心
2017年第3期470-477,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31560086)
