姜黄素联合顺铂对人前列腺癌PC-3细胞株的影响

The influence of Curcumin combined with Cisplatin on human prostate cancer cell line PC-3

目的观察姜黄素联合顺铂(DDP)对人前列腺癌PC-3细胞株抗肿瘤活性作用的影响,并探讨其作用机制。方法采用MTT法检测姜黄素单独或联合顺铂对人前列腺癌PC-3细胞株的抑制率;采用流式细胞术检测不同浓度姜黄素和顺铂单药、联合处理后对人前列腺癌PC-3细胞株凋亡率变化;RT-PCR检测姜黄素单独或联合顺铂对人前列腺癌PC-3细胞株作用后P53的mRNA蛋白表达。结果不同浓度的姜黄素组随着药物浓度增加、作用时间延长,细胞抑制率上升,差异有统计学意义(P<0.05);联合用药组明显高于单一用药组(P<0.05),两药联合具有协同作用;姜黄素和顺铂均促进人前列腺癌PC-3细胞株凋亡,联合用药可显著增加凋亡率;RT-PCR结果显示联合用药组能使P53 mRNA蛋白表达水平较单药组上升。结论姜黄素对人前列腺癌PC-3细胞株具有生长抑制作用,且具有剂量和时间依赖性,其与顺铂联合具有协同抑制作用,其协同作用可能与上调P53 mRNA表达有关。

Objective To observe the effect of Curcumin combined with Cisplatin （DDP） on human prostate cancer cell line PC-3 and explore the mechanism. Methods MTT assay was used to detect the inhibition rate of Curcu-min alone or combination with Cisplatin on human prostate cancer cell line PC-3. Flow cytometry was used to detect the apoptosis rate. RT-PCR method was used to analyze the mRNA expression level of p53. Results The cell inhibition rate increased with the increase of concentration and action time of Curcumin. The effect of the combined treatment group was significantly higher than that of each single medication group （P 〈0. 05） . The combination had a synergistic effect. Both Curcumin and Cisplatin induced the apoptosis of human prostate cancer cell line PC-3 ,and the combination could signif-icantly increase the apoptosis rate （P 〈0.05）. RT-PCR results showed that the expression level of P53 mRNA was in-creased more in the combination group compared with that in the single drug group （P 〈0. 0 5 ） . Conclusion Curcumin can inhibit the growth of human prostate cancer cell line PC-3 with a dose- and time-dependent manner. The combination with Cisplatin have a synergistic inhibitory effect,which may be related to the upregulation of P53 expression.