HPLC法同时测定感冒止咳胶囊中4种成分的含量

Simultaneous Determination of Four Constituents in Ganmao Zhike Capsules by HPLC

目的:建立HPLC法同时测定感冒止咳胶囊中绿原酸、黄芩苷、连翘苷、葛根素的含量。方法:采用Agilent公司的ZORBAX SB-Aq C_(18)柱(4.6 mm×250 mm,5μm);流动相为0.1%磷酸-乙腈,梯度洗脱;体积流量1.0 mL·min^(-1);检测波长为黄芩苷277 nm、连翘苷277 nm、绿原酸327 nm、葛根素250 nm;柱温30℃。结果:绿原酸、黄芩苷、连翘苷、葛根素的线性范围分别为1.672~418.0、2.010~502.4、1.715~428.8、1.754~438.4μg·mL^(-1)(r≥0.9996)。4种成分的平均回收率在92.9%~99.4%。结论:该方法简便、准确、灵敏、重复性好,可用于感冒止咳胶囊4种成分的含量测定。

Objective： To establish an HPLC method for simultaneous determination of the contents of chlorogenic acid, baicalin, forsythin and puerarin in Ganmao Zhike capsules. Methods： The analysis was conducted on an Agilent column ZORBAX SB-Aq （4.6mm×2 5 0mm, 5 μm） at 30 T1, using 0.1% （ v/ v） phos-phoric acid-acetonitrile as the mobile phase in a gradient elution mode with the flow rate at 1.0 mL-min-1. The detection wavelengths were set at 277 nm for forsythin and baicalin, 327 nm for chlorogenic acid and 250 nm for puerarin, respectively. Results： The linear ranges of chlorogenic acid, baicalin, forsythin and puerarin were 1.672-418.0 μg ^-mL·-1, 2.010-502.4μg ^-mL·-1, 1.715-428.8 μg ^-mL·-1 and 1.754-438.4 μg ^-mL·-1（ r=0.9996）, respectively. The average recoveries were between 92.9%-99.4%. Conclusion： The method is accurate, simple and convenient with good repeatability, which can be used for quality control of Ganmao Zhike capsules.