牛磺酸对锰染毒大鼠纹状体神经组织细胞钙平衡调节的影响

Effect of the mediation of the taurine on striatum tissue Ca^2＋homeostasis in manganese exposed rats

目的 观察锰和活性钙调素含量及Na^＋-K^＋-ATPase、Ca^2＋-Mg^2＋-ATPase活力变化,探讨牛磺酸对锰染毒大鼠神经毒性的影响.方法 取SPF级SD健康雄性大鼠156只,随机分为对照组,10、15、20 mg/kg 3个剂量的染锰组和9个牛磺酸干预组,牛横酸干预剂量分别为100、150、200 mg/kg,每组12只大鼠.干预12周后,分离出大鼠纹状体,分别进行锰和活性钙调素含量及Na^＋-K^＋-ATPase、Ca^2＋-Mg^2＋-ATPase活力的测定.结果 各染锰组和牛磺酸干预组纹状体锰含量均高于对照组,差异均有统计学意义（均P〈0.05）;10 mg/kg染锰组活性钙调素含量高于对照组（P〈0.05）,150、200 mg/kg的牛磺酸干预明显降低10 mg/kg锰染毒大鼠纹状体活性钙调素含量,差异均有统计学意义（均P〈0.05）;染锰组大鼠纹状体Na^＋-K^＋-ATPase、Ca^2＋-Mg^2＋-ATPase活力水平均低于对照组（均P〈0.05）,150 mg/kg牛磺酸干预明显增加10 mg/kg锰染毒大鼠纹状体Na^＋-K^＋-ATPase活力水平（P〈0.05）,150 mg/kg牛磺酸干预明显增加15 mg/kg锰染毒大鼠纹状体Ca^2＋-Mg^2＋-ATPase活力水平（P〈0.05）,200 mg/kg牛磺酸干预明显增加10 mg/kg锰染毒大鼠纹状体Ca^2＋-Mg^2＋-ATPase活力水平（P〈0.05）.结论 锰可降低大鼠纹状体中Na^＋-K^＋-ATPase、Ca^2＋-Mg^2＋-ATPase活力,低剂量锰可致活性钙调素含量升高;牛磺酸在一定程度上可对抗锰所致的Na^＋-K^＋-ATPase、Ca^2＋-Mg^2＋-ATPase活力降低作用,对锰所致的活性钙调素异常变化发挥调节作用.

Objective To elucidate the effect of taurine on neurotoxicity induced by Mn by investigating activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase and content of Mn and active calmodulin in manganese exposed rats. Methods 156 male SD rats were randomly divided into 1 control group, 3 manganese exposed groups （10, 15, and 20 mg/kg respectively）, and 9 taurine intervened groups based on orthogonal design （doses of taurine intervention were 100,150, and 200 mg/kg respectively）, with 12 rats in each group. After 12 weeks of exposure, all rats were decapitated and corpus striatums were removed, activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase and content of Mn and active calmodulin were analyzed. Results The corpus striatum Mn content of the 3 dose groups exposed to Mn and 9 taurine intervened groups were significantly higher than that of the control group （P〈0.05）. Active calmodulin content in 10 mg/kg manganese exposed group was significantly higher than that of the control group （P〈0.05）. 150 and 200 mg/kg of taurine could decrease active calmodulin content of the group exposed to 10 mg/kg of Mn （P〈0.05）. The corpus striatum activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase of the 3 dose groups exposed to Mn were significantly lower than that of the control group （P〈0.05）. 150 mg/kg of taurine could increase activities of Na^＋-K^＋-ATPase of the group exposed to 10 mg/kg of Mn（P〈0.05）. 150 and 200 mg/kg of taurine could respectively improve activities of Ca^2＋-Mg^2＋-ATPase of the group exposed to 15,10 mg/kg of Mn （P〈0.05）. Conclusion Mn can decrease the rats corpus striatum activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase, effect level of active calmodulin in relation to dose of Mn, to a certain extent, taurine could regulate activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase and improve the level of active calmodulin.