不同浓度舒芬太尼对脂多糖诱导的外周血中白细胞介素6和肿瘤坏死因子-α浓度的影响

The effect of different concentrations of sufentanil on interleukin-6 and tumor necrosis factor-or concentrations in peripheralblood induced by lipopolysaccharide

目的探讨不同浓度舒芬太尼对脂多糖（1ipopolysaccharide，LPS）诱导的正常人离体外周血中IL-6和TNF-α浓度的影响。方法30例正常志愿者，采用完全随机设计分组法分为对照组（A组）、LPS组（B组）、舒芬太尼组（C组）、舒芬太尼和LPS组（D组），采集正常志愿者外周静脉血5m1，收集在抗凝试管内，准备好标记过（A、B、C1、C2、C3、D1、D2、D3）的8个试管，用高精度加样器每管加入每例志愿者100I．l的静脉血。在8个试管中依次加入以下的8组试剂：A组加入生理盐水，B组加入LPS100mg／L，C组（Cl组、C2组、C3组）分别加入舒芬太尼0．05、0．50、5．00μg／L，D组（D1组、D2组、D3组）分别加入舒芬太尼0．05、0．50、5．00μg/L和LPS100mg／L。用人IL石和TNF-a ELISA试剂盒检测处理后血样上清液中IL-6和TNF吨的浓度。结果A组和C组外周血中IL-6、TNF-α浓度差异无统计学意义（P〉0．05）；与A组和C组比较，B组和D组外周血中IL-6、TNF-α浓度显著增加（P〈0．05）；与B组比较，D组外周血中IL-6、TNF-α浓度显著降低（P〈0．05）；与Dl组比较，D2组、D3组外周血中IL-6、TNF-α浓度降低（P〈0．05）；与D2组比较，D3组外周血中IL-6、TNF-α浓度降低（P〈0．05）。结论LPS可促进人离体外周血中炎性因子IL-6和TNF-α浓度的增加；舒芬太尼能抑制LPS诱导的人离体外周血中IL-6和TNF咄浓度的增加，且呈浓度依赖性；舒芬太尼对正常人离体外周血中IL-6和TNF-α浓度均无影响。

Objective Investigating the effect of different concentration of sufentanil on IL-6 and TNF-α concentrations in human peripheral blood induced by lipopolysaccharide （LPS）. Methods Every blood sample were collected from 30 healthy volunteers. Using a completely randomized design grouping method. Samples were randomized assigned in eight groups. Venous blood 5 ml from every volunteers were collected. 100 μl of the venous blood from every sample were put in eight ethylenediaminete- traacetic acid-vitro ready tubes labeled （A, B, C1, C2, C3, DI, D2, D3） by using precision pipette. Groups are： group A for the saline group （control）, group B joined LPS 100 mg/L, group C includes C1, C2, C3, respectively fentanyl 0.05, 0.50, 5.00 μg/L, group D includes the D1, D2, D3 fentanyl 0.05, 0.50, 5.00 μg/L and LPS 100 mg/L, respectively. The IL-6 and TNF-α ELISA kit were used to detect supematant concentrations of IL-6 and TNF-α. Results Group A and group C in peripheral blood IL-6 and TNF-α concentration were not significantly different （P〉0.05）. Plus LPS of group B and group D of IL-6, of TNF-α concentrations were significantly increased（P〈0.05）. Group D and group B, the peripheral blood of IL-6 and TNF-α concentrations were significantly lower （P〈O.05）. Group D2, D3 peripheral IL-6 and TNF-α concentration reduction are greater than the group D1 （P〈O.05）. Group D3 peripheral blood IL-6 and TNF-α concentration were greater than group D2 reduction （P〈0,05）. Conclusions LPS can promote isolated human peripheral blood inflammatory cytokine IL-6 and TNF-α concentration increasing. Sufentanil inhibited LPS-induced, IL-6 and TNF-α concentration increase in a dose-dependent manner from the peripheral blood. Sufentanil had no effect on IL-6 and TNF-α concentration the peripheral blood drawn away from people.