IL-1、IL-10 mRNA在异常胆液质载体溃疡性结肠炎大鼠结肠组织中的表达

Expression of IL-1 and IL-10 mRNAs in colonic tissue of ulcerative colitis rats with abnormal sapra syndrome

目的探讨异常胆液质载体溃疡性结肠炎大鼠结肠组织中炎症相关因子白介素(interleukin,IL)-1a、IL-1b、IL-10在异常胆液质载体溃疡性结肠炎病证发生、发展中的作用.方法根据维吾尔医学体液理论建立异常胆液质载体证候模型的基础上,采用2,4,6-三硝基苯磺酸(trinitro-benzene-sulfonic acid,TNBS)/乙醇法构建异常胆液质载体溃疡性结肠炎(ulcerative colitis,UC)病证大鼠模型,并进行鉴定,应用实时荧光定量逆转录聚合酶链反应(quantitative reverse transcriptionpolymerase chain reaction,qRT-PCR)方法 ,检测正常组与异常胆液质载体UC病证模型组(模型组)大鼠结肠组织中I L-1a、I L-1b、IL-10 mRNA表达水平,并分析其表达差异.结果模型鉴定与HE染色结果显示,模型组大鼠体征、症状、结肠黏膜损伤等均符合异常胆液质载体UC病证模型的判定标准;qRT-PCR结果显示,与正常组比较,模型组大鼠结肠组织中IL-1a、IL-1b、IL-10的mRNA表达水平均上调,差异有统计学意义(P<0.05).结论异常胆液质载体UC病证模型组大鼠结肠组织中存在炎症相关因子平衡紊乱.

AIM To investigate the role of interleukin（IL）-1a, IL-1b and IL-10 in the pathogenesis and development of ulcerative colitis in rats with abnormal sapra syndrome.METHODS A rat model of abnormal sapra syndrome was used to develop UC using trinitro-benzenesulfonic acid（TNBS）/ethanol. Quantitative reverse transcription-polymerasechain reaction（qRT-PCR） was used to detect the differences in IL-1a, IL-1b and IL-10 mRNA expression levels in the colonic tissues between normal rats（control group） and UC rats with abnormal sapra syndrome（model group）. RESULTS Rat signs, symptoms and colonic mucosa damage suggested that UC with abnormal sapra syndrome was successfully induced.qRT-PCR results showed that compared with the normal group, the expression of IL-1a, IL-1b and IL-10 mRNAs was significantly upregulated in the model group（P 〈 0.05）. CONCLUSION Imbalance of infl ammatory factors may occur in colon tissue of UC rats with abnormal sapra syndrome.