摘要
为研究无溶剂系统脂肪酶催化玉米胚芽油与辛酸酸解制备功能性脂质的可能性及反应条件,以玉米胚芽油为原料,比较6种脂肪酶(Lipozyme RM IM、Lipozyme TL IM、Novozym 435、Lipase AK、Lipase AY和Newlase F)对无溶剂体系中固定化脂肪酶催化玉米胚芽油与辛酸酸解制备MLM结构脂质的影响。研究结果表明:所选6种脂肪酶中,来自米黑根毛霉的固定化脂肪酶Lipozyme RM IM的催化效果最好。当玉米胚芽油与辛酸的比率为1∶4(物质的量比)、脂肪酶量为12%(底物质量分数)、反应时间16 h、反应温度50℃时,玉米胚芽油中辛酸插入率最高。在无溶剂系统,利用Lipozyme RM IM脂肪酶催化玉米胚芽油与辛酸进行酸解反应,可制备高品质的MLM型功能性脂质。
In order to produce functional MLM-type structured Lipid with maize germ oil in solvent free system was investigated.Six commercial lipases from different sources(Lipozyme RM IM、Lipozyme TL IM、Novozym 435、Lipase AK、Lipase AY and Newlase F) were compared for their ability to incorporate caprylic acid into the maize germ oil in solvent free system.Of the six lipases that were tested in the initial screening,Lipozyme RM IM from Rhizomucor miehei resulted in the highest incorporation of caprylic acid into maize germ oil.This enzyme was further studied for the effect of mole ratio of oil and caprylic acid,enzyme load,reaction time and reaction temperature on the incorporation of caprylic acid into maize germ oil.Incorporation of caprylic acid was higher when reactions were carried with 12% lipase of the total weight of substrates with a 1∶4 mole ratio of oil and caprylic acid,time course and temperature for synthesis MLMtype structured lipids were 16 h and 50 ℃.Lipase-catalyzed acidolysis of grape seed oil with caprylic acid could produce high quality MLM-type structured lipids in solvent free system.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第1期77-83,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金面上项目(31671787)
国家自然科学基金青年项目(31401559)
重庆高校创新团队建设计划资助项目(CXTDX201601040)
关键词
玉米胚芽油
辛酸
脂肪酶
酸解反应
MLM型结构脂质
maize germ oil
caprylic acid
lipase
acidolysis reaction
MLM-type structured lipids