妇科再造胶囊对多囊卵巢综合征患者miRNA-383和miRNA-320表达的影响研究

Study on the effect of gynecological reengineering capsule on miRNA-383 and miRNA-320 of polycystic ovarian syndrome

目的:研究妇科再造胶囊对多囊卵巢综合征(PCOS)患者miRNA-383和miRNA-320表达的影响。方法:选择2014年5月至2015年10月在本院诊断为PCOS患者80例,随机分为对照组和观察组(各40例),对照组采用炔雌醇环丙孕酮片治疗,观察组采用妇科再造胶囊治疗,治疗前和治疗6个月后采集患者血清测定睾酮(TSTO)、黄体生成素(LH)、卵泡刺激素(FSH)含量,采集卵泡颗粒细胞测定miR-383和miR-320的表达量。结果:两组治疗6个月后血清中TSTO、LH含量显著低于治疗前,FSH含量显著高于治疗前,组间比较差异无统计学意义。对照组治疗6个月后颗粒细胞中miR-383和miR-320的表达量无改变;观察组显著降低,差异有统计学意义(P<0.05)。结论:妇科再造胶囊治疗PCOS的可能分子靶点是卵泡颗粒细胞miR-383和miR-320。

Objective： To study the effect of gynecological reengineering capsule on miRNA-383 and miRNA-320 of polycystic ovarian syndrome（PCOS）. Methods：A total of 80 consecutives of PCOS received diane-35 combined with gynecological reengineering capsule therapy in our hospital during May 2014 to October 2015. The levels of serum testosterone （TSTO）, luteinizing hormone （LH）, follicle stimulation hormone （FSH）, expression of miR-383 and miR-320 in follicular granulosa cells were compared before and 6 months after the treatment. Results：The serum levels of TSTO, LH and miR-383, miR-320 in follicular granulosa cells were all significantly lower after the treatment, FSH level was significantly higher. The serum androgen level was positive to miR-383 and miR-320 expression by Pearson correlation analysis, the correlation coefficient r being 0. 682 and 0. 714. Conclusion：Inhibition of miR-383 and miR-320 expression in follicular granulosa cells is a potential molecular target of gynecological reengineering capsule treating PCOS.