加味逍遥方中20种无机元素的分布规律及相关性研究

Distribution and correlation of 20 kinds of inorganic elements in Jiawei Xiaoyao Formula

目的建立加味逍遥方中无机元素的微波消解-电感耦合等离子体质谱(ICP-MS)分析方法,对不同批次加味逍遥提取物和组方药材中无机元素进行比较和分析。方法采用微波消解法对样品进行前处理,ICP-MS法对加味逍遥提取物中铁、锌、铜、锰、镍、钒、铬、钴、硒、锶、铯、砷、镉、银、汞、铅、铀20种无机元素的进行了定量,并应用SPSS 22.0软件对结果进行统计学分析。结果加味逍遥提取物中20种无机元素之间具有一定的相关性,应用主成分分析选出4个主因子,得出Na、Mg、Al、V、Ni、Cu、Se、Sr、Cs、Mn、Hg、Fe为加味逍遥提取物的特征元素;组方药材中主要含有Mg、Al、Mn、Fe;10味药材因其药用部位不同无机元素水平差异较大,同种元素水平从根及根茎类、皮类、全草类、果实种子类、菌类依次降低。结论无机元素的水平变化不仅能表征提取工艺过程的稳定性,而且能为中药复方配伍规律研究提供实验依据。

Objective To study the distribution and correlation of 20 kinds of inorganic elements （Na, Mg, Al, V, Cr, Co, Fe, Ni, Cu, Zn, Mn, As, Se, Cd, Cs, Sr, Ag, Hg, Pb, and U） in Jiawei Xiaoyao Formula （JXF）. Methods The levels of 20 inorganic elements in 10 kinds of herbal substances were determined by ICP-MS with microwave digestion for sample preparation, and the results were analyzed with SPSS22.0. Results There were some correlations among the inorganic elements in JXF; Four main factors were selected by principal component analysis （PCA）. The PCA results showed that No, Mg, Al, V, Ni, Cu, Se, Sr, Cs, Mn, Hg, and Fe might be the characteristic elements in JXF; There were Mg, Al, Mn, and Fe elements in 10 herbal drugs, the contents of inorganic elements in herbal drug were different because the medicinal parts were different, and the decreasing order was roots and rhizomes, skin type, all grass, fruit seeds, and fungi. Conclusion Contents of inorganic elements can not only characterize the stability of extraction process, and can provide experimental evidence for the study of traditional Chinese medicine compound compatibility law.