丹栀逍遥散对多囊卵巢大鼠高雄激素血症雄激素、瘦素及其受体表达的影响

Effects of Danzhi Xiaoyao Powder on Androgen,Leptin and Leptin Receptor in Polycentric Ovary Hyperandrogenism Rats

目的观察丹栀逍遥散对多囊卵巢综合征伴高雄激素血症大鼠雄激素、瘦素及其受体的影响。方法利用脱氢表雄酮诱导建立多囊卵巢大鼠高雄激素血症模型,随机设立丹栀逍遥组、达英组、模型组、空白对照组,予以相应药物干预。实验结束后观察卵巢形态学变化;酶联免疫法测定血清雄激素水平;ELISA法检测血清瘦素;免疫组化测定卵巢瘦素受体的表达。结果与模型组相比,丹栀逍遥散可显著降低大鼠血清瘦素、睾酮、游离睾酮水平值,减少卵巢瘦素受体阳性表达,差异均有统计学意义(P<0.01)。结论丹栀逍遥散可降低多囊卵巢高雄激素血症大鼠血清瘦素、雄激素水平值,减少卵巢瘦素受体阳性表达,进而降低瘦素生物利用度及雄激素活性,从而改善多囊卵巢综合征高雄激素血症和排卵障碍。

Objective To observe the effect of Danzhi Xiaoyao Powder （DZXYP） on the expression of androgen, leptin and leptin receptor （LEPR） in polycentric ovary （PCO） hyperandrogenism rats. Methods The PCO Hyperandrogenism model rats induced by dehydroepiandrosterone （DHEA） were randomly divided into DZXYP group, Diane group, model group and blank control group. The morphologic changes were observed after the experiment. The levels of androgen were determined by radio-immunity. Serum leptin was determined by ELISA. LEPR in the tissues of ovaries was assessed with immunohistochem-istry method. Results Danzhi Xiaoyao Powder could reduce the values of leptin, testosterone, free testosterone, and the expres-sion of LEPR obviously, the differences were statistically significant （P〈0.01）. Conclusion DZXYP could reduce the levels of leptin, androgen and decrease the expression of LEPR of PCO hyperandrogenism rats. Then it could decrease the bioavailabili-ty of leptin and androgenic activities to improve high serum-T and ovulation barrier of polycentric ovary syndrome.